Cytokine expression profile of UCB- versus AB-stimulated T-cell supernatants
. | UCB . | AB . |
---|---|---|
Cytokines, pg/mL | ||
IL-2 | 2122 ± 208 | 1815 ± 73 |
IL-3 | 127 ± 19 | 532 ± 30 |
IL-4 | 5.7 ± 1 | 125 ± 15 |
IL-5 | 9 ± 2.8 | 147 ± 3 |
IL-10 | 232 ± 18 | 20 310 ± 70 |
IL-13 | <LDL | 410 ± 2 |
IL-1β | 2 ± 0 | 18.9 ± 2.7 |
GM-CSF | 168 ± 11 | 1450 ± 186 |
Chemokines, pg/mL | ||
RANTES | 118 ± 18 | 452 ± 28 |
MIG | 17.7 ± 0.9 | 74 ± 32 |
MIP-1α | 664 ± 77 | 2479 ± 253 |
IL-8 | 441 ± 17 | 827 ± 65 |
. | UCB . | AB . |
---|---|---|
Cytokines, pg/mL | ||
IL-2 | 2122 ± 208 | 1815 ± 73 |
IL-3 | 127 ± 19 | 532 ± 30 |
IL-4 | 5.7 ± 1 | 125 ± 15 |
IL-5 | 9 ± 2.8 | 147 ± 3 |
IL-10 | 232 ± 18 | 20 310 ± 70 |
IL-13 | <LDL | 410 ± 2 |
IL-1β | 2 ± 0 | 18.9 ± 2.7 |
GM-CSF | 168 ± 11 | 1450 ± 186 |
Chemokines, pg/mL | ||
RANTES | 118 ± 18 | 452 ± 28 |
MIG | 17.7 ± 0.9 | 74 ± 32 |
MIP-1α | 664 ± 77 | 2479 ± 253 |
IL-8 | 441 ± 17 | 827 ± 65 |
Purified CD4+ T cells from one UCB and adult each, were stimulated for 20 hours and supernatants were analyzed in duplicates for cytokine expression using fluorescent microspheres. Numbers are average of duplicate samples (± standard deviation). LDL indicates lower detection level. Representative of 3 performed experiments.