Table 5.

Cytokine expression profile of UCB- versus AB-stimulated T-cell supernatants




UCB

AB
Cytokines, pg/mL   
IL-2   2122 ± 208   1815 ± 73  
IL-3   127 ± 19   532 ± 30  
IL-4   5.7 ± 1   125 ± 15  
IL-5   9 ± 2.8   147 ± 3  
IL-10   232 ± 18   20 310 ± 70  
IL-13   <LDL   410 ± 2  
IL-1β   2 ± 0   18.9 ± 2.7  
GM-CSF   168 ± 11   1450 ± 186  
Chemokines, pg/mL   
RANTES   118 ± 18   452 ± 28  
MIG   17.7 ± 0.9   74 ± 32  
MIP-1α   664 ± 77   2479 ± 253  
IL-8
 
441 ± 17
 
827 ± 65
 



UCB

AB
Cytokines, pg/mL   
IL-2   2122 ± 208   1815 ± 73  
IL-3   127 ± 19   532 ± 30  
IL-4   5.7 ± 1   125 ± 15  
IL-5   9 ± 2.8   147 ± 3  
IL-10   232 ± 18   20 310 ± 70  
IL-13   <LDL   410 ± 2  
IL-1β   2 ± 0   18.9 ± 2.7  
GM-CSF   168 ± 11   1450 ± 186  
Chemokines, pg/mL   
RANTES   118 ± 18   452 ± 28  
MIG   17.7 ± 0.9   74 ± 32  
MIP-1α   664 ± 77   2479 ± 253  
IL-8
 
441 ± 17
 
827 ± 65
 

Purified CD4+ T cells from one UCB and adult each, were stimulated for 20 hours and supernatants were analyzed in duplicates for cytokine expression using fluorescent microspheres. Numbers are average of duplicate samples (± standard deviation). LDL indicates lower detection level. Representative of 3 performed experiments.

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