Induction of selected genes in INA-6 and XG-1 strains expressing mutated EpoR/gp130 chimeras after 1-hour Epo stimulation as determined by real-time PCR
. | IL-6 . | wtEg . | ΔY1/4/5 . | ΔY1 . | ΔY2-5 . | ΔY1-5 . |
|---|---|---|---|---|---|---|
| INA-6 cells | ||||||
| junB | 12.1 ± 3.1 | 14.7 ± 1.1 | 6.6 ± 1.5 | 15.7 ± 1.8 | 2.5 ± 0.5 | 2.5 ± 0.9 |
| SOCS3 | 24.7 ± 1.3 | 281.8 ± 59.3 | 136.1 ± 41.7 | 277.5 ± 72.0 | 4.7 ± 1.4 | 5.8 ± 1.5 |
| Pim-1 | 8.2 ± 0.7 | 4.1 ± 0.2 | 2.9 ± 0.3 | 3.9 ± 0.4 | 1.1 ± 0.2 | 1.2 ± 0.1 |
| HB-EGF | 5.3 ± 0.7 | 5.3 ± 0.6 | 3.5 ± 0.5 | 4.8 ± 0.4 | 1.4 ± 0.3 | 1.4 ± 0.5 |
| SGK | 14.0 ± 0.6 | 10.5 ± 3.3 | 6.3 ± 1.1 | 7.1 ± 1.5 | 1.4 ± 0.4 | 0.9 ± 0.1 |
| GADD45β | 6.0 ± 2.6 | 9.0 ± 2.5 | 5.4 ± 0.8 | 11.3 ± 2.4 | 3.2 ± 1.4 | 3.1 ± 1.3 |
| ETR101 | 2.1 ± 0.5 | 3.3 ± 0.9 | 2.3 ± 0.6 | 2.0 ± 0.5 | 1.6 ± 0.1 | 1.0 ± 0.2 |
| c-fos | 1.8 ± 0.4 | 5.9 ± 1.2 | 4.7 ± 1.2 | 2.3 ± 0.7 | 4.7 ± 1.6 | 3.8 ± 0.9 |
| XG-1 cells | ||||||
| junB | 5.6 ± 0.4 | 7.7 ± 0.6 | 5.6 ± 1.1 | 6.9 ± 1.0 | 2.5 ± 0.5 | 1.6 ± 0.4 |
| SOCS3 | 29.8 ± 2.1 | 108.8 ± 41.2 | 27.3 ± 3.7 | 54.6 ± 23.2 | 0.5 ± 0.2 | 2.6 ± 0.1 |
| Pim-1 | 3.3 ± 0.3 | 3.1 ± 0.5 | 2.5 ± 0.5 | 4.3 ± 1.2 | 1.2 ± 0.5 | 1.5 ± 0.5 |
| HB-EGF | 5.7 ± 0.5 | 6.7 ± 1.7 | 4.4 ± 1.0 | 4.6 ± 0.4 | 1.7 ± 0.2 | 1.8 ± 0.1 |
| SGK | 2.3 ± 0.3 | 2.8 ± 0.6 | 3.0 ± 0.6 | 2.9 ± 0.4 | 1.8 ± 0.4 | 1.4 ± 0.1 |
| GADD45β | 6.8 ± 1.5 | 12.3 ± 3.1 | 4.4 ± 0.4 | 6.6 ± 1.0 | 1.9 ± 0.6 | 1.3 ± 0.3 |
| ETR101 | 1.7 ± 0.2 | 3.0 ± 0.5 | 2.0 ± 0.3 | 1.8 ± 0.3 | 3.0 ± 0.5 | 1.5 ± 0.2 |
| c-fos | 9.7 ± 1.6 | 5.0 ± 1.1 | 2.9 ± 0.5 | 3.3 ± 0.5 | 1.7 ± 0.6 | 1.6 ± 0.1 |
. | IL-6 . | wtEg . | ΔY1/4/5 . | ΔY1 . | ΔY2-5 . | ΔY1-5 . |
|---|---|---|---|---|---|---|
| INA-6 cells | ||||||
| junB | 12.1 ± 3.1 | 14.7 ± 1.1 | 6.6 ± 1.5 | 15.7 ± 1.8 | 2.5 ± 0.5 | 2.5 ± 0.9 |
| SOCS3 | 24.7 ± 1.3 | 281.8 ± 59.3 | 136.1 ± 41.7 | 277.5 ± 72.0 | 4.7 ± 1.4 | 5.8 ± 1.5 |
| Pim-1 | 8.2 ± 0.7 | 4.1 ± 0.2 | 2.9 ± 0.3 | 3.9 ± 0.4 | 1.1 ± 0.2 | 1.2 ± 0.1 |
| HB-EGF | 5.3 ± 0.7 | 5.3 ± 0.6 | 3.5 ± 0.5 | 4.8 ± 0.4 | 1.4 ± 0.3 | 1.4 ± 0.5 |
| SGK | 14.0 ± 0.6 | 10.5 ± 3.3 | 6.3 ± 1.1 | 7.1 ± 1.5 | 1.4 ± 0.4 | 0.9 ± 0.1 |
| GADD45β | 6.0 ± 2.6 | 9.0 ± 2.5 | 5.4 ± 0.8 | 11.3 ± 2.4 | 3.2 ± 1.4 | 3.1 ± 1.3 |
| ETR101 | 2.1 ± 0.5 | 3.3 ± 0.9 | 2.3 ± 0.6 | 2.0 ± 0.5 | 1.6 ± 0.1 | 1.0 ± 0.2 |
| c-fos | 1.8 ± 0.4 | 5.9 ± 1.2 | 4.7 ± 1.2 | 2.3 ± 0.7 | 4.7 ± 1.6 | 3.8 ± 0.9 |
| XG-1 cells | ||||||
| junB | 5.6 ± 0.4 | 7.7 ± 0.6 | 5.6 ± 1.1 | 6.9 ± 1.0 | 2.5 ± 0.5 | 1.6 ± 0.4 |
| SOCS3 | 29.8 ± 2.1 | 108.8 ± 41.2 | 27.3 ± 3.7 | 54.6 ± 23.2 | 0.5 ± 0.2 | 2.6 ± 0.1 |
| Pim-1 | 3.3 ± 0.3 | 3.1 ± 0.5 | 2.5 ± 0.5 | 4.3 ± 1.2 | 1.2 ± 0.5 | 1.5 ± 0.5 |
| HB-EGF | 5.7 ± 0.5 | 6.7 ± 1.7 | 4.4 ± 1.0 | 4.6 ± 0.4 | 1.7 ± 0.2 | 1.8 ± 0.1 |
| SGK | 2.3 ± 0.3 | 2.8 ± 0.6 | 3.0 ± 0.6 | 2.9 ± 0.4 | 1.8 ± 0.4 | 1.4 ± 0.1 |
| GADD45β | 6.8 ± 1.5 | 12.3 ± 3.1 | 4.4 ± 0.4 | 6.6 ± 1.0 | 1.9 ± 0.6 | 1.3 ± 0.3 |
| ETR101 | 1.7 ± 0.2 | 3.0 ± 0.5 | 2.0 ± 0.3 | 1.8 ± 0.3 | 3.0 ± 0.5 | 1.5 ± 0.2 |
| c-fos | 9.7 ± 1.6 | 5.0 ± 1.1 | 2.9 ± 0.5 | 3.3 ± 0.5 | 1.7 ± 0.6 | 1.6 ± 0.1 |
Real-time PCR data from 2 independent experiments were obtained from INA-6 and XG-1 strains expressing mutated EpoR/gp 130 chimeras treated as described in Figure 5. The values for 1-hour IL-6 stimulation of unmanipulated cells are given for comparison (IL-6). Data are expressed as mean fold induction ± standard deviation from at least 3 measurements. Values from cytokine-starved wild-type Eg (wtEg) were taken as baseline.