Candidate epitopes predicted by computer algorithms
HLA restricted . | Parameter submitted . | . | Result predicted‡ . | . | . | . | Rank in whole pp65 . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Range, aa† . | HLA type . | Position, aa . | Sequence . | Length . | Score . | . | ||||
| A*0207 | 504-561 | A*0201 | 522-530 | RIFAELEGV | 9 | 39.8 | 12 | ||||
| A*1101 | 473-513 | A*1101 | 501-509 | ATVQGQNLK | 9 | 1.5 | 3 | ||||
| B*1501 | 202-234 | B*1501§ | 215-223 | KMQVIGDQY§∥ | 9 | 117§ | 4 | ||||
| B*4001 | 225-249 | B60 | 232-240 | CEDVPSGKL | 9 | 176 | 1 | ||||
| B*4001 | 262-287 | B60 | 267-275 | HERNGFTVL | 9 | 160 | 2 | ||||
| B*4002 | 262-287 | B61 | 267-275 | HERNGFTVL | 9 | 8 | 6 | ||||
| B*4006 | 504-561 | B40 | 525-534 | AELEGVWQPA | 10 | 80 | 1 | ||||
| B*4403 | 346-380 | B*4403 | 364-373 | SEHPTFTSQY | 10 | 720 | 1 | ||||
| B*5101 | 504-561 | B*5101 | 547-555 | LPGPCIAST | 9 | 14.3 | 25 | ||||
| Cw*0102 | 1-40 | Cw*0102§ | 7-15 | RCPEMISVL§ | 9 | 200§ | 3 | ||||
| Cw*0401 | 336-378 | Cw*0401 | 341-349 | QYDPVAALF | 9 | 216 | 4 | ||||
HLA restricted . | Parameter submitted . | . | Result predicted‡ . | . | . | . | Rank in whole pp65 . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Range, aa† . | HLA type . | Position, aa . | Sequence . | Length . | Score . | . | ||||
| A*0207 | 504-561 | A*0201 | 522-530 | RIFAELEGV | 9 | 39.8 | 12 | ||||
| A*1101 | 473-513 | A*1101 | 501-509 | ATVQGQNLK | 9 | 1.5 | 3 | ||||
| B*1501 | 202-234 | B*1501§ | 215-223 | KMQVIGDQY§∥ | 9 | 117§ | 4 | ||||
| B*4001 | 225-249 | B60 | 232-240 | CEDVPSGKL | 9 | 176 | 1 | ||||
| B*4001 | 262-287 | B60 | 267-275 | HERNGFTVL | 9 | 160 | 2 | ||||
| B*4002 | 262-287 | B61 | 267-275 | HERNGFTVL | 9 | 8 | 6 | ||||
| B*4006 | 504-561 | B40 | 525-534 | AELEGVWQPA | 10 | 80 | 1 | ||||
| B*4403 | 346-380 | B*4403 | 364-373 | SEHPTFTSQY | 10 | 720 | 1 | ||||
| B*5101 | 504-561 | B*5101 | 547-555 | LPGPCIAST | 9 | 14.3 | 25 | ||||
| Cw*0102 | 1-40 | Cw*0102§ | 7-15 | RCPEMISVL§ | 9 | 200§ | 3 | ||||
| Cw*0401 | 336-378 | Cw*0401 | 341-349 | QYDPVAALF | 9 | 216 | 4 | ||||
The different region between the shortest positive deletion mutant and the longest negative one (Figure 2) with a 10 aa extension to the N-terminus was submitted.
All results except for the marked ones§ were predicted by “HLA Peptide Binding Predictions” on BIMAS website.23
Predicted by “HLA Epitope binding prediction” on the website of University of Oklahoma Health Sciences Center.24
By computer algorithm on BIMAS website, the peptide was assigned 3rd rank (score = 4) within the submitted range and 21st rank in the full-length pp65 gene, respectively.