The membrane proximal domain of NKp46 recognizes the HA of different viruses
Virus . | HA type . | NKp44-Ig . | NKp46-Ig . | NKp46D2-Ig . | NKp46D1-Ig . | NKp30-Ig . | CD99-Ig . |
|---|---|---|---|---|---|---|---|
| Uninfected | — | 7 | 3 | 4 | 2 | 2 | 0 |
| SV | HA-NA | 350 | 77 | 95 | 8 | 7 | 0.5 |
| Beijing | H1N1 | 128 | 44 | 77 | 7 | 4 | 0 |
| X-127 | H1N1 | 220 | 107 | 160 | 5 | 6 | 1 |
| New Caledonia | H1N1 | 518 | 315 | 420 | 5 | 10 | 0 |
| Sydney | H3N2 | 120 | 58 | 100 | 10 | 8 | 1 |
| Moscow | H3N2 | 24 | 20 | 50 | 8 | 8 | 0 |
| Yamanashi | B strain | 117 | 37 | 51 | 8 | 12 | 0 |
Virus . | HA type . | NKp44-Ig . | NKp46-Ig . | NKp46D2-Ig . | NKp46D1-Ig . | NKp30-Ig . | CD99-Ig . |
|---|---|---|---|---|---|---|---|
| Uninfected | — | 7 | 3 | 4 | 2 | 2 | 0 |
| SV | HA-NA | 350 | 77 | 95 | 8 | 7 | 0.5 |
| Beijing | H1N1 | 128 | 44 | 77 | 7 | 4 | 0 |
| X-127 | H1N1 | 220 | 107 | 160 | 5 | 6 | 1 |
| New Caledonia | H1N1 | 518 | 315 | 420 | 5 | 10 | 0 |
| Sydney | H3N2 | 120 | 58 | 100 | 10 | 8 | 1 |
| Moscow | H3N2 | 24 | 20 | 50 | 8 | 8 | 0 |
| Yamanashi | B strain | 117 | 37 | 51 | 8 | 12 | 0 |
721.221 cells were incubated overnight with or without influenza viruses that express different types of HA. Cells (50 000/well) were washed and stained with the various Ig fusion proteins (5 μg/well) followed by PE-conjugated goat antihuman Fc. The level of infection was determined by staining with human sera derived from individuals immunized with a trivalent influenza vaccine. Median fluorescence intensity (MFI) numbers were rounded to the nearest whole number after subtracting background staining (of PE-conjugated goat antihuman Fc). These results represent one experiment of 15 performed. — indicates no infection.