Definitive progenitor analysis of E10.5 YS
Genotype . | G1-G2+/+tg . | G1-G2+/+tg . | G1-G2+/- . | G1-G2-/- . | G1-G2-/- tg . | G1+/-G2-/- tg . | G1+G2-/- . | G1+/-G2+/+ . |
|---|---|---|---|---|---|---|---|---|
| Colony no. | 585 ± 73 | 385 ± 96 | 188 ± 24 | 2 ± 1.6 | 1 ± 1.2 | 2 ± 1.7 | 8 ± 3 | 369 ± 17 |
Genotype . | G1-G2+/+tg . | G1-G2+/+tg . | G1-G2+/- . | G1-G2-/- . | G1-G2-/- tg . | G1+/-G2-/- tg . | G1+G2-/- . | G1+/-G2+/+ . |
|---|---|---|---|---|---|---|---|---|
| Colony no. | 585 ± 73 | 385 ± 96 | 188 ± 24 | 2 ± 1.6 | 1 ± 1.2 | 2 ± 1.7 | 8 ± 3 | 369 ± 17 |
Shown are colony numbers from E10.5 YS progenitor analysis. Genotypes were performed by Southern hybridization with embryo parts. E10.5 YS were dissociated with trypsin/EDTA (ethylenediaminetetetraacetic acid) and are divided into 5 methylcellulose dishes. Colony numbers were counted after 7 days. Data are shown as mean ± SD.