Apoptosis and cell cycle analysis in normal CD34+ hematopoietic progenitor cells after 24 or 48 hours of exposure to imatinib
. | 24 h . | . | . | 48 h . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Imatinib, μM . | Apoptosis, % . | G1, % . | S/G2/M, % . | Apoptosis, % . | G1, % . | S/G2/M, % . | ||||
| 0 | 7.8 ± 3.4 | 70.2 ± 3.0 | 20.4 ± 3.7 | 5.0 ± 1.1 | 70.7 ± 5.4 | 20.4 ± 4.4 | ||||
| 0.3125 | 10.1 ± 4.0 | 69.5 ± 2.5 | 16.6 ± 2.0) | 8.6 ± 1.8 | 71.2 ± 6.5 | 18.5 ± 2.5 | ||||
| 0.625 | 9.8 ± 4.9 | 73.7 ± 3.8 | 14.8 ± 1.0 | 10.5 ± 3.4 | 69.7 ± 7.9 | 17.5 ± 2.8 | ||||
| 1.25 | 9.6 ± 4.9 | 73.0 ± 4.9 | 15.2 ± 1.8 | 10.7 ± 4.1 | 70.7 ± 7.6 | 15.6 ± 4.2 | ||||
| 2.5 | 9.4 ± 4.4 | 73.3 ± 6.0 | 14.6 ± 2.2 | 10.5 ± 2.7 | 70.3 ± 6.0 | 16.4 ± 3.3 | ||||
| 5 | 10.9 ± 5.5 | 73.3 ± 6.0 | 13.8 ± 2.3 | 13.1 ± 3.8 | 69.2 ± 8.0 | 14.4 ± 4.1 | ||||
| 10 | 12.0 ± 7.2 | 71.7 ± 9.3 | 13.1 ± 2.3 | 21.5 ± 11.3 | 62.8 ± 10.8 | 12.3 ± 4.3 | ||||
. | 24 h . | . | . | 48 h . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Imatinib, μM . | Apoptosis, % . | G1, % . | S/G2/M, % . | Apoptosis, % . | G1, % . | S/G2/M, % . | ||||
| 0 | 7.8 ± 3.4 | 70.2 ± 3.0 | 20.4 ± 3.7 | 5.0 ± 1.1 | 70.7 ± 5.4 | 20.4 ± 4.4 | ||||
| 0.3125 | 10.1 ± 4.0 | 69.5 ± 2.5 | 16.6 ± 2.0) | 8.6 ± 1.8 | 71.2 ± 6.5 | 18.5 ± 2.5 | ||||
| 0.625 | 9.8 ± 4.9 | 73.7 ± 3.8 | 14.8 ± 1.0 | 10.5 ± 3.4 | 69.7 ± 7.9 | 17.5 ± 2.8 | ||||
| 1.25 | 9.6 ± 4.9 | 73.0 ± 4.9 | 15.2 ± 1.8 | 10.7 ± 4.1 | 70.7 ± 7.6 | 15.6 ± 4.2 | ||||
| 2.5 | 9.4 ± 4.4 | 73.3 ± 6.0 | 14.6 ± 2.2 | 10.5 ± 2.7 | 70.3 ± 6.0 | 16.4 ± 3.3 | ||||
| 5 | 10.9 ± 5.5 | 73.3 ± 6.0 | 13.8 ± 2.3 | 13.1 ± 3.8 | 69.2 ± 8.0 | 14.4 ± 4.1 | ||||
| 10 | 12.0 ± 7.2 | 71.7 ± 9.3 | 13.1 ± 2.3 | 21.5 ± 11.3 | 62.8 ± 10.8 | 12.3 ± 4.3 | ||||
DNA content was determined according to Nicoletti et al21 and analyzed by flow cytometry. The percentage of apoptotic cells was defined as the percentage of CD34+ cells containing decreased levels of DNA (hypodiploid peak). Data are expressed as mean ± SD of 3 independent experiments.