Table 1.

List of primers used for qRT-PCR




Forward

Reverse
GAPDH (82F/182R)   5′-AGGGCTGCCTTCTCTTGTGAC-3′   5′-TGGGTAGAATCATACTGGAACATGTAG-3′  
HFE (582F/682R)   5′-TGGGCAAGATCACCTTGAATT-3′   5′-GGATCCTGTGCTCTTCCCACT-3′  
Transferrin (1392F/1492R)   5′-GGCATCAGACTCCAGCATCA-3′   5′-GCAGGCCCATAGGGATGTT-3′  
Hepcidin (165F/252R)   5′-TGACAGTGCGCTGCTGATG-3′   5′-GGAATTCTTACAGCATTTACAGCAGA-3′  
TfR2* (1446F/1546R)   5′-AGCTGGGACGGAGGTGACTT-3′   5′-TCCAGGCTCACGTACACAACAG-3′  
Ceruloplasmin (2945F/3025R)   5′-ATGTGATGGCTATGGGCAATG-3′   5′-TTCCCCTGTGCTTGTATTGGA-3′  
Nramp1* (1302F/1402R)   5′-ATCCTGCCCACTGTGTTGGT-3′   5′-GCGAAGGGCAGCAGTAGACT-3′  
TfR1 (2227F/2302R)   5′-AGTGGTCGCTGGGTGTGATT-3′   5′-CCTTCAGGCATACAGCTCAATTG-3′  
FPN1 (434F/534R)   5′-GGTGGTGGCAGGCTCTGT-3′   5′-TTTGAACCACCAGGGACGTC-3′  
DMT1 (1007F/1107R)   5′-ATAGCAGCAGCCCCCATG-3′   5′-AGGCCCGAAGTAACATCCAA-3′  
Hephaestin (3119F/3233R)
 
5′-GGCACAGTTACAGGGCAGATG-3′
 
5′-ACATGGTCAGTAACGTGGCAGT-3′
 



Forward

Reverse
GAPDH (82F/182R)   5′-AGGGCTGCCTTCTCTTGTGAC-3′   5′-TGGGTAGAATCATACTGGAACATGTAG-3′  
HFE (582F/682R)   5′-TGGGCAAGATCACCTTGAATT-3′   5′-GGATCCTGTGCTCTTCCCACT-3′  
Transferrin (1392F/1492R)   5′-GGCATCAGACTCCAGCATCA-3′   5′-GCAGGCCCATAGGGATGTT-3′  
Hepcidin (165F/252R)   5′-TGACAGTGCGCTGCTGATG-3′   5′-GGAATTCTTACAGCATTTACAGCAGA-3′  
TfR2* (1446F/1546R)   5′-AGCTGGGACGGAGGTGACTT-3′   5′-TCCAGGCTCACGTACACAACAG-3′  
Ceruloplasmin (2945F/3025R)   5′-ATGTGATGGCTATGGGCAATG-3′   5′-TTCCCCTGTGCTTGTATTGGA-3′  
Nramp1* (1302F/1402R)   5′-ATCCTGCCCACTGTGTTGGT-3′   5′-GCGAAGGGCAGCAGTAGACT-3′  
TfR1 (2227F/2302R)   5′-AGTGGTCGCTGGGTGTGATT-3′   5′-CCTTCAGGCATACAGCTCAATTG-3′  
FPN1 (434F/534R)   5′-GGTGGTGGCAGGCTCTGT-3′   5′-TTTGAACCACCAGGGACGTC-3′  
DMT1 (1007F/1107R)   5′-ATAGCAGCAGCCCCCATG-3′   5′-AGGCCCGAAGTAACATCCAA-3′  
Hephaestin (3119F/3233R)
 
5′-GGCACAGTTACAGGGCAGATG-3′
 
5′-ACATGGTCAGTAACGTGGCAGT-3′
 

F indicates forward; R, reverse.

*

Primers were designed on the basis of the corresponding mouse mRNA sequences because of the lack of available rat sequences.

DMT1 primers can amplify both IRE and non-IRE forms of DMT1 cDNA.

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