A3D8 induces differentiation antigens in both empty vector- and ASp27-transfected NB4 and THP-1 cells
MFI of CD15 in NB4 cells . | . | . | . | % CD14+ THP-1 cells . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Empty vector . | . | ASp27 . | . | Empty vector . | . | ASp27 . | . | ||||||
| +IgG1 . | +A3D8 . | +IgG1 . | +A3D8 . | +IgG1 . | +A3D8 . | +IgG1 . | +A3D8 . | ||||||
| 50 ± 5 | 121 ± 7 | 31 ± 4 | 88 ± 5 | < 5% | 34% ± 8% | < 5% | 41% ± 5% | ||||||
MFI of CD15 in NB4 cells . | . | . | . | % CD14+ THP-1 cells . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Empty vector . | . | ASp27 . | . | Empty vector . | . | ASp27 . | . | ||||||
| +IgG1 . | +A3D8 . | +IgG1 . | +A3D8 . | +IgG1 . | +A3D8 . | +IgG1 . | +A3D8 . | ||||||
| 50 ± 5 | 121 ± 7 | 31 ± 4 | 88 ± 5 | < 5% | 34% ± 8% | < 5% | 41% ± 5% | ||||||
NB4 cells were infected with empty vector- or Asp27-retrovirus-containing medium and treated 2 days later with A3D8 (time 0) for 3 days, as described in “Materials and methods,” and labeled by using FITC-conjugated mAb to CD14 or CD15 (as described in the first footnote of Table 1). Because all NB4 cells display CD15, only the mean fluorescence intensities (MFIs) vary. Data are means ± 1 SD calculated from 4 samples. This is 1 experiment representative of 2. Data of A3D8-treated samples are significantly different from IgG1-treated ones (P < .05, Mann-Whitney test).