RFX PCR primer sequence and conditions
Primer . | Sequence . | Annealing temperature . | Cycles . |
|---|---|---|---|
| RFX5 sense | 5′-AAGCTGTATCTCTACCTTCAG-3′ | 59°C | 35 |
| RFX5 antisense | 5′-TTTCAGGATCCGCTCTGCCCA-3′ | 59°C | 35 |
| RFXAP sense | 5′-CACCCTGCTAGTGATGCAACCC-3′ | 66°C | 37 |
| RFXAP antisense | 5′-GAGTAGGTCTTGCAGGAGATCC-3′ | 66°C | 37 |
| RFXB sense | 5′-GCCAGATCGCTGAGGGTCCG-3′ | 66°C | 35 |
| RFXB antisense | 5′-CCGGCAGGCGGCCTTCACTC-3′ | 66°C | 35 |
Primer . | Sequence . | Annealing temperature . | Cycles . |
|---|---|---|---|
| RFX5 sense | 5′-AAGCTGTATCTCTACCTTCAG-3′ | 59°C | 35 |
| RFX5 antisense | 5′-TTTCAGGATCCGCTCTGCCCA-3′ | 59°C | 35 |
| RFXAP sense | 5′-CACCCTGCTAGTGATGCAACCC-3′ | 66°C | 37 |
| RFXAP antisense | 5′-GAGTAGGTCTTGCAGGAGATCC-3′ | 66°C | 37 |
| RFXB sense | 5′-GCCAGATCGCTGAGGGTCCG-3′ | 66°C | 35 |
| RFXB antisense | 5′-CCGGCAGGCGGCCTTCACTC-3′ | 66°C | 35 |
The MgCl2 concentration for all primers was 3 mM.