Intravital microscopy parameters
Treatment . | No. of mice (venules) . | Venule diameter, μm . | No. of rolling WBCs per minute . | No. of adherent WBCs per 100 μm . | Wall shear rates, IVM 1; s-1 . | Wall shear rates, IVM 2; s-1 . | Survival: IVM 1 + IVM 2 . |
|---|---|---|---|---|---|---|---|
| PBS | 5 (43) | 21 ± 1 | 31 ± 8 | 11.5 ± 1.7 | 436 ± 58 | — | 0 of 5 |
| IVIG 1 mg/kg | 6 (39) | 21 ± 1 | 36 ± 4 | 10.8 ± 2.3 | 454 ± 54 | — | 1 of 6 |
| IVIG 10 mg/kg | 3 (39) | 20 ± 1 | 37 ± 13 | 7.4 ± 2.0 | 423 ± 56 | — | 0 of 3 |
| IVIG 200 mg/kg | 5 (31) | 20 ± 1 | 48 ± 5 | 6.3 ± 1.3* | 627 ± 76 | 417 ± 77 | 3 of 5 |
| IVIG 400 mg/kg | 5 (32) | 20 ± 1 | 37 ± 9 | 6.3 ± 1.5* | 765 ± 87† | 405 ± 48 | 4 of 5 |
| Albumin 400 mg/kg | 5 (41) | 19 ± 1 | 18 ± 5 | 11.1 ± 1.4 | 386 ± 93 | — | 0 of 5 |
| Fab 200 mg/kg | 5 (30) | 21 ± 1 | 29 ± 11 | 10.6 ± 1.0 | 456 ± 81 | — | 0 of 5 |
| Fc 200 mg/kg | 5 (30) | 18 ± 1 | 34 ± 9 | 8.3 ± 2.1 | 662 ± 151 | — | 1 of 5 |
Treatment . | No. of mice (venules) . | Venule diameter, μm . | No. of rolling WBCs per minute . | No. of adherent WBCs per 100 μm . | Wall shear rates, IVM 1; s-1 . | Wall shear rates, IVM 2; s-1 . | Survival: IVM 1 + IVM 2 . |
|---|---|---|---|---|---|---|---|
| PBS | 5 (43) | 21 ± 1 | 31 ± 8 | 11.5 ± 1.7 | 436 ± 58 | — | 0 of 5 |
| IVIG 1 mg/kg | 6 (39) | 21 ± 1 | 36 ± 4 | 10.8 ± 2.3 | 454 ± 54 | — | 1 of 6 |
| IVIG 10 mg/kg | 3 (39) | 20 ± 1 | 37 ± 13 | 7.4 ± 2.0 | 423 ± 56 | — | 0 of 3 |
| IVIG 200 mg/kg | 5 (31) | 20 ± 1 | 48 ± 5 | 6.3 ± 1.3* | 627 ± 76 | 417 ± 77 | 3 of 5 |
| IVIG 400 mg/kg | 5 (32) | 20 ± 1 | 37 ± 9 | 6.3 ± 1.5* | 765 ± 87† | 405 ± 48 | 4 of 5 |
| Albumin 400 mg/kg | 5 (41) | 19 ± 1 | 18 ± 5 | 11.1 ± 1.4 | 386 ± 93 | — | 0 of 5 |
| Fab 200 mg/kg | 5 (30) | 21 ± 1 | 29 ± 11 | 10.6 ± 1.0 | 456 ± 81 | — | 0 of 5 |
| Fc 200 mg/kg | 5 (30) | 18 ± 1 | 34 ± 9 | 8.3 ± 2.1 | 662 ± 151 | — | 1 of 5 |
Wall shear rates were calculated based on the Poiseuille law for a newtonian fluid (γ = 8(Vmean/Dv) where Dv is the venule diameter and Vmean is estimated from the centerline RBC velocities (Vmean = VRBC/1.6). The numbers of rolling and adherent WBCs were determined by 3 counts of 1 minute per venule. WBCs were considered adherent if they remained stationary for more than 30 seconds and were counted over a venular segment of 100 μm. Several venules were recorded per animal, and the averaged values are reported as mean ± SEM. IVM 1, first intravital microscopy viewing period (before TNF-α); IVM 2, second intravital microscopy viewing period (after TNF-α). — indicates that the number of live mice in the group was too small for a reliable determination to be made.
P < .05
P < .01 compared with the PBS group