Oligonucleotides used in mutation analysis
Gene (accession no.) . | Primer sequence . | Use . | PCR product, bp . |
|---|---|---|---|
| CD95 5′ UTR (al157394) | AAGAGTGACACACAGGTGTTC | P + S | 478 |
| AAGGCCCAAGAAAAGCAAGTC | P + S | ||
| CD95 exon 8 (al157394) | CCTTCTTAATCACTTAATCTAGC | P + S | 336 |
| CTGCCTGATAAATGCTTATGCTG | P + S | ||
| CD95 exon 9 (m67454) | TGGGAATTTCATTTAGAAAAACA | P + S | 413 |
| TACTCAGAACTGAATTTGTTGT | P + S | ||
| PAX5 (af386791) | AGGGACCTCAGAAGCATCGAGGCC | P + S | 932 |
| TGAAAAAGGCGCCATCGGAGTAG | P + S | ||
| CACTGTAAGCACGACCCG | S | ||
| CGGGTCGTGCTTACAGTG | S | ||
| RhoH/TTF (af386789) | GCTTTTACTCTAGGCCAAACATCG | P + S | 875 |
| CTTCTACCGACACTTCGCATTCTT | P + S | ||
| CTGAGGTGGTTTGATTTGG | S | ||
| CCAAATCAAACCACCTCAG | S | ||
| c-MYC exon 1 (x00364) | CACCGGCCCTTTATAATGCG | P + S | 1302 |
| ACGATTCCAGGAGAATCGGA | P + S | ||
| CTTGCCGCATCCACGAAAC | S | ||
| GGAGAGGAGAAGGCAGAG | S | ||
| CCCAGAGAGCAATTAACAC | S | ||
| GTGTTGTAAGTTCCAGTGC | S | ||
| c-MYC intron 1 (x00364) | TCTCTAGAGGTGTTAGGACG | P + S | 865 |
| AGAGCTATCCCCTAAAGCGG | P + S | ||
| GCTGGCAAAAGGAGTGTTG | S | ||
| CTCCCAACCTTCCCTCTC | S | ||
| c-MYC exon 2 (x00364) | CCGCTGGTTCACTAAGTGCG | P + S | 1167 |
| GGATGGGAGGAAACGCTAAAG | P + S | ||
| CAGCGAGGATATCTGGAAG | S | ||
| CTTGTACCTGCAGGATCTG | S | ||
| CTTCCAGATATCCTCGCTG | S | ||
| CAGATCCTGCAGGTACAAG | S | ||
| PIM1 (af386792) | TTCTCCGGCGTCATTAGGC | P + S | 1025 |
| CGTTTGTTAGGTTAAGCCGC | P + S | ||
| GCCAGCTGAACCTGTAATG | S | ||
| CATTACAGGTTCAGCTGGC | S |
Gene (accession no.) . | Primer sequence . | Use . | PCR product, bp . |
|---|---|---|---|
| CD95 5′ UTR (al157394) | AAGAGTGACACACAGGTGTTC | P + S | 478 |
| AAGGCCCAAGAAAAGCAAGTC | P + S | ||
| CD95 exon 8 (al157394) | CCTTCTTAATCACTTAATCTAGC | P + S | 336 |
| CTGCCTGATAAATGCTTATGCTG | P + S | ||
| CD95 exon 9 (m67454) | TGGGAATTTCATTTAGAAAAACA | P + S | 413 |
| TACTCAGAACTGAATTTGTTGT | P + S | ||
| PAX5 (af386791) | AGGGACCTCAGAAGCATCGAGGCC | P + S | 932 |
| TGAAAAAGGCGCCATCGGAGTAG | P + S | ||
| CACTGTAAGCACGACCCG | S | ||
| CGGGTCGTGCTTACAGTG | S | ||
| RhoH/TTF (af386789) | GCTTTTACTCTAGGCCAAACATCG | P + S | 875 |
| CTTCTACCGACACTTCGCATTCTT | P + S | ||
| CTGAGGTGGTTTGATTTGG | S | ||
| CCAAATCAAACCACCTCAG | S | ||
| c-MYC exon 1 (x00364) | CACCGGCCCTTTATAATGCG | P + S | 1302 |
| ACGATTCCAGGAGAATCGGA | P + S | ||
| CTTGCCGCATCCACGAAAC | S | ||
| GGAGAGGAGAAGGCAGAG | S | ||
| CCCAGAGAGCAATTAACAC | S | ||
| GTGTTGTAAGTTCCAGTGC | S | ||
| c-MYC intron 1 (x00364) | TCTCTAGAGGTGTTAGGACG | P + S | 865 |
| AGAGCTATCCCCTAAAGCGG | P + S | ||
| GCTGGCAAAAGGAGTGTTG | S | ||
| CTCCCAACCTTCCCTCTC | S | ||
| c-MYC exon 2 (x00364) | CCGCTGGTTCACTAAGTGCG | P + S | 1167 |
| GGATGGGAGGAAACGCTAAAG | P + S | ||
| CAGCGAGGATATCTGGAAG | S | ||
| CTTGTACCTGCAGGATCTG | S | ||
| CTTCCAGATATCCTCGCTG | S | ||
| CAGATCCTGCAGGTACAAG | S | ||
| PIM1 (af386792) | TTCTCCGGCGTCATTAGGC | P + S | 1025 |
| CGTTTGTTAGGTTAAGCCGC | P + S | ||
| GCCAGCTGAACCTGTAATG | S | ||
| CATTACAGGTTCAGCTGGC | S |
P indicates PCR; S, sequencing.