Effects of adipocyte-differentiated MSCs on apoptosis and proliferation of NB4 cells
Treatment and culture condition . | Annexin V positive, % . | Viable cells × 105/mL . |
|---|---|---|
| FCS 10% | ||
| Control, cultured alone | 23.6 ± 4.8 | 8.6 ± 0.6 |
| Cocultured with adipocyte | 21.5 ± 2.0 | 9.1 ± 1.1 |
| Cocultured with adipocyte + chimeric OB-R | 22.5 ± 3.8 | 8.5 ± 1.3 |
| Cocultured with adipocyte + leptin antibody | 22.5 ± 2.9 | 8.4 ± 1.5 |
| FCS 1% | ||
| Control, cultured alone | 62.3 ± 4.7 | 4.1 ± 0.7 |
| Cocultured with adipocyte | 47.1 ± 4.7* | 5.2 ± 1.2 |
| Cocultured with adipocyte + chimeric OB-R | 54.2 ± 3.3*† | 4.5 ± 1.1 |
| Cocultured with adipocyte + leptin antibody | 54.4 ± 2.5*† | 4.1 ± 0.5 |
| Doxorubicin, 10 ng/mL; FCS 10% | ||
| Control, cultured alone | 46.9 ± 1.2 | 3.4 ± 0.3 |
| Cocultured with adipocyte | 38.8 ± 3.0* | 5.3 ± 0.2‡ |
| Cocultured with adipocyte + chimeric OB-R | 43.8 ± 2.3*† | 4.2 ± 0.3†‡ |
| Cocultured with adipocyte + leptin antibody | 42.4 ± 2.4*† | 3.8 ± 0.4†‡ |
| ATRA, 1 mM; FCS 10% | ||
| Control, cultured alone | 26.8 ± 5.0 | 4.8 ± 2.6 |
| Cocultured with adipocyte | 24.4 ± 5.1 | 5.0 ± 1.9 |
| Cocultured with adipocyte + chimeric OB-R | 28.8 ± 4.9 | 4.4 ± 2.9 |
| Cocultured with adipocyte + leptin antibody | 26.7 ± 2.0 | 4.5 ± 1.6 |
Treatment and culture condition . | Annexin V positive, % . | Viable cells × 105/mL . |
|---|---|---|
| FCS 10% | ||
| Control, cultured alone | 23.6 ± 4.8 | 8.6 ± 0.6 |
| Cocultured with adipocyte | 21.5 ± 2.0 | 9.1 ± 1.1 |
| Cocultured with adipocyte + chimeric OB-R | 22.5 ± 3.8 | 8.5 ± 1.3 |
| Cocultured with adipocyte + leptin antibody | 22.5 ± 2.9 | 8.4 ± 1.5 |
| FCS 1% | ||
| Control, cultured alone | 62.3 ± 4.7 | 4.1 ± 0.7 |
| Cocultured with adipocyte | 47.1 ± 4.7* | 5.2 ± 1.2 |
| Cocultured with adipocyte + chimeric OB-R | 54.2 ± 3.3*† | 4.5 ± 1.1 |
| Cocultured with adipocyte + leptin antibody | 54.4 ± 2.5*† | 4.1 ± 0.5 |
| Doxorubicin, 10 ng/mL; FCS 10% | ||
| Control, cultured alone | 46.9 ± 1.2 | 3.4 ± 0.3 |
| Cocultured with adipocyte | 38.8 ± 3.0* | 5.3 ± 0.2‡ |
| Cocultured with adipocyte + chimeric OB-R | 43.8 ± 2.3*† | 4.2 ± 0.3†‡ |
| Cocultured with adipocyte + leptin antibody | 42.4 ± 2.4*† | 3.8 ± 0.4†‡ |
| ATRA, 1 mM; FCS 10% | ||
| Control, cultured alone | 26.8 ± 5.0 | 4.8 ± 2.6 |
| Cocultured with adipocyte | 24.4 ± 5.1 | 5.0 ± 1.9 |
| Cocultured with adipocyte + chimeric OB-R | 28.8 ± 4.9 | 4.4 ± 2.9 |
| Cocultured with adipocyte + leptin antibody | 26.7 ± 2.0 | 4.5 ± 1.6 |
NB4 cells were cocultured with adipocyte-differentiated MSC monolayers with or without chimeric OB-R leptin antibody in conditions of reduced serum (1%), with 10 ng/mL doxorubicin or 1 mM ATRA for 72 hours in at least 3 experiments. Results represent the percentage of apoptosis as determined by annexin V labeling and viable cell numbers using the trypan blue exclusion method. Apoptosis and viability of NB4 cells cocultured with adipocytes were compared with cells cultured in medium alone. Statistically significant differences were determined by paired Student ttest; all others were P ≥ .05. Apoptosis and viability of NB4 cells cocultured with adipocytes were compared with cells cocultured with adipocytes in the presence of chimeric OB-R or leptin antibody.
P < .05.
Statistically significant difference demonstrating reversal of the protective effects of adipocyte coculture on apoptosis.
P < .01.