Impaired expression and function of NKp46 on activated NK clones
. | Patient A . | . | Patient B . | . | Patient C . | . | Healthy sister . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | NKp46, MFI . | .221 cells killed, % . | NKp46, MFI . | .221 cells killed, % . | NKp46, MFI . | .221 cells killed, % . | NKp46, MFI . | .221 cells killed, % . | ||||
| NKp46- | 2.2 ± 0.9 (30/30) | 6.7 ± 2.9 (30/30) | 1.7 ± 0.6 (19/49) | 7.2 ± 3.7 (19/49) | 1.8 ± 0.7 (56/70) | 4.8 ± 2.1 (56/70) | N/O | N/O | ||||
| NKp46+ | N/O | N/O | 16.5 ± 6.3 (29/49) | 25 ± 5.9 (29/49) | 13.5 ± 6 (14/70) | 20.9 ± 6 (14/70) | 16.9 ± 5 (40/40) | 26.5 ± 5.3 (40/40) | ||||
. | Patient A . | . | Patient B . | . | Patient C . | . | Healthy sister . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | NKp46, MFI . | .221 cells killed, % . | NKp46, MFI . | .221 cells killed, % . | NKp46, MFI . | .221 cells killed, % . | NKp46, MFI . | .221 cells killed, % . | ||||
| NKp46- | 2.2 ± 0.9 (30/30) | 6.7 ± 2.9 (30/30) | 1.7 ± 0.6 (19/49) | 7.2 ± 3.7 (19/49) | 1.8 ± 0.7 (56/70) | 4.8 ± 2.1 (56/70) | N/O | N/O | ||||
| NKp46+ | N/O | N/O | 16.5 ± 6.3 (29/49) | 25 ± 5.9 (29/49) | 13.5 ± 6 (14/70) | 20.9 ± 6 (14/70) | 16.9 ± 5 (40/40) | 26.5 ± 5.3 (40/40) | ||||
Activated NK clones were prepared from each individual as indicated. Clones were stained for NKp46 expression by means of the 461-G1 mAb in the form of F(ab′)2 and concomitantly tested for cytotoxic activity against .221 cells. NKp46 expression is presented as the mean MFI of different NK clones ± SD. Cytotoxic activity is presented as the mean percentage of .221 cells killed by NK clones. The data represent mean percentage of cells killed ± SD. The number of NK clones included in the analysis out of total NK clones tested in each group are indicated in parentheses.
N/O indicates not observed.