Primers used for RT-PCR
Primer . | Sequence . | Product . |
|---|---|---|
| Collagen type I | S: 5′-TGCTTGAATGTGCTGATGACAGGG-3′ | 414 bp |
| A: 5′-TCCCCTCACCCTCCCAGTAT-3′ | ||
| Osteocalcin | S: 5′-CGCAGCCACCGAGACACCAT-3′ | 405 bp |
| A: 5′-GGGCAAGGGCAAGGGGAAGA-3′ | ||
| Osteopontin | S: 5′-CTAGGCATCACCTGTGCCATACC-3′ | 330 bp |
| A: 5′-CTACTTAGACTACTTGACCAGTGAC-3′ | ||
| Syndecan | S: 5′-CCTTCACACTCCCCACAC-3′ | 409 bp |
| A: 5′-GGCATAGAATTCCTCCTGTTG-3′ | ||
| Perlecan | S: 5′-CATAGAGACCGTCACAGCAAG-3′ | 300 bp |
| A: 5′-ATGAACACCACACTGACAACC-3′ | ||
| Aggrecan | S: 5′-TCAGGAACTGAACTCAGTGG-3′ | 441 bp |
| A: 5′-GCCACTGAGTTCCACAGA-3′ | ||
| Collagen type II | S: 5′-ACGGCGAGAAGGGAGAAGTTG-3′ | 352 bp |
| A: 5′-GGGGGTCCAGGGTTGCCATTG-3′ | ||
| Glial fibrillary acidic protein | S: 5′-GCAGAGATGATGGAGCTCAATGACC-3′ | 266 bp |
| A: 5′-GTTTCATCCTGGAGCTTCTGCCTCA-3′ | ||
| Microtubule-associated protein | S: 5′-TGCCATCTTGGTGCCGA-3′ | 370 bp |
| A: 5′-CTTGACATTACCACCTCCAGGT-3′ | ||
| α-fetoprotein | S: 5′-TGCAGCCAAAGTGAAGAGGGAAGA-3′ | 216 bp |
| A: 5′-CATAGCGAGCAGCCCAAAGAAGAA-3′ | ||
| Tyrosine aminotransferase | S: 5′-TGAGCAGTCTGTCCACTGCCT-3′ | 358 bp |
| A: 5′-ATGTGAATGAGGAGGATCTGAG-3′ | ||
| Albumin | S: 5′-TGC TTG AATGTGCTGATGACAGGG-3′ | 161 bp |
| A: 5′-AAGGCAAGTCAGCAGGCATCTCATC-3′ | ||
| Cytokeratin 18 | S: 5′-TGGTACTCTCCTCAATCTGCTG-3′ | 148 bp |
| A: 5′-CTCTGGATTGACTGTGGAAGT-3′ |
Primer . | Sequence . | Product . |
|---|---|---|
| Collagen type I | S: 5′-TGCTTGAATGTGCTGATGACAGGG-3′ | 414 bp |
| A: 5′-TCCCCTCACCCTCCCAGTAT-3′ | ||
| Osteocalcin | S: 5′-CGCAGCCACCGAGACACCAT-3′ | 405 bp |
| A: 5′-GGGCAAGGGCAAGGGGAAGA-3′ | ||
| Osteopontin | S: 5′-CTAGGCATCACCTGTGCCATACC-3′ | 330 bp |
| A: 5′-CTACTTAGACTACTTGACCAGTGAC-3′ | ||
| Syndecan | S: 5′-CCTTCACACTCCCCACAC-3′ | 409 bp |
| A: 5′-GGCATAGAATTCCTCCTGTTG-3′ | ||
| Perlecan | S: 5′-CATAGAGACCGTCACAGCAAG-3′ | 300 bp |
| A: 5′-ATGAACACCACACTGACAACC-3′ | ||
| Aggrecan | S: 5′-TCAGGAACTGAACTCAGTGG-3′ | 441 bp |
| A: 5′-GCCACTGAGTTCCACAGA-3′ | ||
| Collagen type II | S: 5′-ACGGCGAGAAGGGAGAAGTTG-3′ | 352 bp |
| A: 5′-GGGGGTCCAGGGTTGCCATTG-3′ | ||
| Glial fibrillary acidic protein | S: 5′-GCAGAGATGATGGAGCTCAATGACC-3′ | 266 bp |
| A: 5′-GTTTCATCCTGGAGCTTCTGCCTCA-3′ | ||
| Microtubule-associated protein | S: 5′-TGCCATCTTGGTGCCGA-3′ | 370 bp |
| A: 5′-CTTGACATTACCACCTCCAGGT-3′ | ||
| α-fetoprotein | S: 5′-TGCAGCCAAAGTGAAGAGGGAAGA-3′ | 216 bp |
| A: 5′-CATAGCGAGCAGCCCAAAGAAGAA-3′ | ||
| Tyrosine aminotransferase | S: 5′-TGAGCAGTCTGTCCACTGCCT-3′ | 358 bp |
| A: 5′-ATGTGAATGAGGAGGATCTGAG-3′ | ||
| Albumin | S: 5′-TGC TTG AATGTGCTGATGACAGGG-3′ | 161 bp |
| A: 5′-AAGGCAAGTCAGCAGGCATCTCATC-3′ | ||
| Cytokeratin 18 | S: 5′-TGGTACTCTCCTCAATCTGCTG-3′ | 148 bp |
| A: 5′-CTCTGGATTGACTGTGGAAGT-3′ |