Ferritin content in transfected HeLa cells
Cells . | H-ferritin, ng/mg total protein . | L-ferritin, ng/mg total protein . |
|---|---|---|
| HeLa control | 393 ± 57 | 74 ± 6 |
| HeLa + H-siRNA | 102 ± 36 | 222 ± 4 |
| HeLa + L-siRNA | 384 ± 21 | 16 ± 6 |
| HeLa + H/L-siRNA | 95 ± 5 | 24 ± 3 |
| Clone ΔH1 | 98 ± 44 | 97 ± 7 |
| L-clone dox+ | 438 ± 40 | 82 ± 5 |
| L-clone dox– | 434 ± 34 | 215 ± 55 |
| HeLa control + FAC 1 mM | 2225 ± 270 | 600 ± 31 |
| Clone ΔH1 + FAC 1 mM | 310 ± 20 | 1540 ± 63 |
Cells . | H-ferritin, ng/mg total protein . | L-ferritin, ng/mg total protein . |
|---|---|---|
| HeLa control | 393 ± 57 | 74 ± 6 |
| HeLa + H-siRNA | 102 ± 36 | 222 ± 4 |
| HeLa + L-siRNA | 384 ± 21 | 16 ± 6 |
| HeLa + H/L-siRNA | 95 ± 5 | 24 ± 3 |
| Clone ΔH1 | 98 ± 44 | 97 ± 7 |
| L-clone dox+ | 438 ± 40 | 82 ± 5 |
| L-clone dox– | 434 ± 34 | 215 ± 55 |
| HeLa control + FAC 1 mM | 2225 ± 270 | 600 ± 31 |
| Clone ΔH1 + FAC 1 mM | 310 ± 20 | 1540 ± 63 |
H-and L-ferritin levels are shown in the siRNA-transfected HeLa cells and control cells, in the clone ΔH1, and in the repressed (dox+) and derepressed (dox–) L-clone. The HeLa cells and the clone ΔH1 were evaluated also after treatment with 1 mM FAC for 18 hours. Ferritin content was determined using specific ELISA assays. Data are expressed as nanograms of ferritin per milligram of total proteins. Means and SD from at least 3 independent experiments are shown.