Results of screening assays for binding of recombinant FGFs to fibrinogen
Constructs . | Total bound, %* . | Specific bound, %† . |
|---|---|---|
| FGF-2 (2222) | 100 | 100 |
| FGF-1 (1111) | 3 ± 2 | 1 ± 1 |
| 2212 | 2 ± 1 | 0 |
| 2211 | 5 ± 3 | 0 |
| 2221 | 9 ± 4 | 0 |
| 1121 | 6 ± 2 | 0 |
| 1122 | 45 ± 9 | 99 ± 15 |
| 2122 | 64 ± 21 | 99 ± 21 |
| 1112 | 5 ± 3 | 0 |
| 1222 | 38 ± 12 | 87 ± 18 |
| 2111 | 6 ± 2 | 0 |
| ACD‡ | 3 ± 1 | 8 ± 4 |
| BCD‡ | 4 ± 1 | 0 |
| ABD‡ | 1 ± 1 | 1 ± 1 |
| ABC‡ | 1 ± 1 | 0 |
| FGF-2_ID§∥ | 86 ± 6 | 100 ± 9 |
| FGF-2_IS∥ | 94 ± 8 | 100 ± 7 |
| FGF-2_2D∥ | 77 ± 17 | 79 ± 26 |
| FGF-2_2S∥ | 77 ± 14 | 83 ± 19 |
| FGF-2_3D∥ | 5 ± 1 | 23 ± 9 |
| FGF-2_3S∥ | 84 ± 6 | 100 ± 13 |
Constructs . | Total bound, %* . | Specific bound, %† . |
|---|---|---|
| FGF-2 (2222) | 100 | 100 |
| FGF-1 (1111) | 3 ± 2 | 1 ± 1 |
| 2212 | 2 ± 1 | 0 |
| 2211 | 5 ± 3 | 0 |
| 2221 | 9 ± 4 | 0 |
| 1121 | 6 ± 2 | 0 |
| 1122 | 45 ± 9 | 99 ± 15 |
| 2122 | 64 ± 21 | 99 ± 21 |
| 1112 | 5 ± 3 | 0 |
| 1222 | 38 ± 12 | 87 ± 18 |
| 2111 | 6 ± 2 | 0 |
| ACD‡ | 3 ± 1 | 8 ± 4 |
| BCD‡ | 4 ± 1 | 0 |
| ABD‡ | 1 ± 1 | 1 ± 1 |
| ABC‡ | 1 ± 1 | 0 |
| FGF-2_ID§∥ | 86 ± 6 | 100 ± 9 |
| FGF-2_IS∥ | 94 ± 8 | 100 ± 7 |
| FGF-2_2D∥ | 77 ± 17 | 79 ± 26 |
| FGF-2_2S∥ | 77 ± 14 | 83 ± 19 |
| FGF-2_3D∥ | 5 ± 1 | 23 ± 9 |
| FGF-2_3S∥ | 84 ± 6 | 100 ± 13 |
Total binding was measured in the absence of cold fibrinogen and is expressed as a percentage of recombinant FGF-2.
Specific binding = (total binding - nonspecific binding) / (total binding × 100).
A, B, C, and D refer to cassettes in FGF-2.
The numbers 1, 2, and 3 refer to areas of site-directed mutagenesis in Figure 2A.
D indicates the deletion of the sequence indicated in Figure 2A, and S indicates substitution of FGF-2 sequence for the corresponding residues in FGF-1.