Down-regulation of Bcl-XL prevents growth of subcutaneous ALK+ tumor xenografts
. | . | . | Tumorigenicity in nude mice . | . | . | ||
|---|---|---|---|---|---|---|---|
| . | Growth properties in vitro . | . | Tumors/no. of injections . | . | Dox treatment, d . | ||
| Cells . | −DOX . | +DOX . | −DOX . | +DOX . | . | ||
| N/A.pEV | + | + | 12/12 | 12/12 | 21 | ||
| N/A.pAS | + | − | 12/12 | 3/12 | 21 | ||
. | . | . | Tumorigenicity in nude mice . | . | . | ||
|---|---|---|---|---|---|---|---|
| . | Growth properties in vitro . | . | Tumors/no. of injections . | . | Dox treatment, d . | ||
| Cells . | −DOX . | +DOX . | −DOX . | +DOX . | . | ||
| N/A.pEV | + | + | 12/12 | 12/12 | 21 | ||
| N/A.pAS | + | − | 12/12 | 3/12 | 21 | ||
In vitro growth and in vivo tumorigenic potential of N/A.pEV and N/A.pAS cells. Cells (5 × 105) were cultured for 4 days in absence/presence of 1 μg/mL Dox. Cell viability was evaluated by acridine orange staining as indicated in Figure 6B. N/A.pEV and N/A.pAS cells (20 × 106) were injected subcutaneously in nude mice. At 24 hours after injection, half of each group was maintained in the presence of Dox (0.2 mg/mL) in the drinking water and treatment lasted for 21 days. Mice were monitored daily for the appearance of tumors.