Table 1. Sequence of the forward and... | American Society of Hematology

Table 1.

Sequence of the forward and reverse primers used in Q-PCR for some of the transcripts identified in the subtracted library of FB-HSPCs

Transcript, gene	Forward primer 5′ 3′	Reverse primer 5′ 3′	Amplicon size, bp
CDK6	CACGAACAGACAGAGAACCA	CGGTGTGAATGAAGAAAGTCC	169
CENPF	AGAACCCACCACGAAATCC	TGCCTTCACTGGACCTTACA	169
ETS2	CCAAGAGAGGAGTGACCCAG	CAGGAGAAACTGCCACAGC	114
FB29	CTTGGGTCAGAGCAGAGGTC	CAGCAGGTTTGGAACTGGA	150
FB83	TCCAGCAACCTTCCATCTCT	TGAGCCAAGAAAGCCATC	184
FB136	TTCCCAAAGTAACAGCCAGTC	TTCATCCTCAGTTTCACCAAA	152
FB248	TCACTTTATCATCAGCCAGCA	TTCTTGGTCTCGCCTACTCC	159
GAPDH	TGCACCACCAACTGCTTAGC	GGCATGGACTGTGGTCATGAC	100
HMBS	GGCAATGCGGCTGCAA	GGGTACCCACGCGAATCAG	80
LMO2	TACTGGCACGAGGACTGC	CTTTCACCCGCATTGTCAT	190
LZTF1	TCTACTCAGGGTTCAGGAGCA	TTGATTTGGTCATTCTTCTTGG	120
MTCO-1	CCTGACTGGCATTGTATTAGC	TTTTGGCGTAGGTTTGGTCT	179
MYCN	TTGTGTGTTCCAAGTTTCCAA	CCACCTCTCATTACCCAGGA	185
NFKB1	TGAGTCCTGCTCCTTCCAA	CTTCGGTGTAGCCCATTTGT	105
Rac1	GTGAATCTGGGCTTATGGGA	GATGGGAGTGTTGGGACAGT	189
RALBP1	GAAGCAGTATTTGCGAGACC	TGGCAGTTCTTTGAGTAAACG	133
RCC1	TGGGTGAGAATGTGATGGAG	GCAGCCGAAGGAATAGACC	137
RNF4	AAGGCACCAAAGAGCACAAT	TTCATCTCCAGCAGTTTCCA	159
TIF1	AGTCATTCGTTGCCCAGTTT	CTTCTGCGTTGTCCTCACAG	152
TRIM28	AATGATGCCCAGAAGGTGAC	TTGAGGTCCCACTGAAACTT	245
WEE1	TGAAGAGGGCGATAGTCGTT	GCACTTGTGGTATCCGAGGT	188

Transcript, gene	Forward primer 5′ 3′	Reverse primer 5′ 3′	Amplicon size, bp
CDK6	CACGAACAGACAGAGAACCA	CGGTGTGAATGAAGAAAGTCC	169
CENPF	AGAACCCACCACGAAATCC	TGCCTTCACTGGACCTTACA	169
ETS2	CCAAGAGAGGAGTGACCCAG	CAGGAGAAACTGCCACAGC	114
FB29	CTTGGGTCAGAGCAGAGGTC	CAGCAGGTTTGGAACTGGA	150
FB83	TCCAGCAACCTTCCATCTCT	TGAGCCAAGAAAGCCATC	184
FB136	TTCCCAAAGTAACAGCCAGTC	TTCATCCTCAGTTTCACCAAA	152
FB248	TCACTTTATCATCAGCCAGCA	TTCTTGGTCTCGCCTACTCC	159
GAPDH	TGCACCACCAACTGCTTAGC	GGCATGGACTGTGGTCATGAC	100
HMBS	GGCAATGCGGCTGCAA	GGGTACCCACGCGAATCAG	80
LMO2	TACTGGCACGAGGACTGC	CTTTCACCCGCATTGTCAT	190
LZTF1	TCTACTCAGGGTTCAGGAGCA	TTGATTTGGTCATTCTTCTTGG	120
MTCO-1	CCTGACTGGCATTGTATTAGC	TTTTGGCGTAGGTTTGGTCT	179
MYCN	TTGTGTGTTCCAAGTTTCCAA	CCACCTCTCATTACCCAGGA	185
NFKB1	TGAGTCCTGCTCCTTCCAA	CTTCGGTGTAGCCCATTTGT	105
Rac1	GTGAATCTGGGCTTATGGGA	GATGGGAGTGTTGGGACAGT	189
RALBP1	GAAGCAGTATTTGCGAGACC	TGGCAGTTCTTTGAGTAAACG	133
RCC1	TGGGTGAGAATGTGATGGAG	GCAGCCGAAGGAATAGACC	137
RNF4	AAGGCACCAAAGAGCACAAT	TTCATCTCCAGCAGTTTCCA	159
TIF1	AGTCATTCGTTGCCCAGTTT	CTTCTGCGTTGTCCTCACAG	152
TRIM28	AATGATGCCCAGAAGGTGAC	TTGAGGTCCCACTGAAACTT	245
WEE1	TGAAGAGGGCGATAGTCGTT	GCACTTGTGGTATCCGAGGT	188