Annotation of sequenced clones identified from the FB-subtracted library
Clone . | Accession no. . | Name of gene . | Annotation . | Domain(s) . |
|---|---|---|---|---|
| FB11 | BC034041 | Homo sapiens, LIM domain only 2 | TF/DNB | LIM |
| FB271 | BC042483 | Homo sapiens, leucine zipper transcription factor-like 1 | TF/DNB | LIM |
| FB70, 305 | NM_005378 | Homo sapiens MYCN mRNA | TF/DNB | HLH, PFAM |
| FB83, 197, 245 | BC028689 | Homo sapiens transcriptional intermediary factor 1 (TIF 1), mRNA | TF/DNB | BROMO, PHD, BBC, B-BOX, RING |
| FB63 | NM_005762 | Homo sapiens tripartite motif-containing 28 | TF/DNB | BROMO, PHD, BBC, B-BOX, RING |
| FB109, 194 | J04102 | Human ETS2 mRNA | TF/DNB | ETS |
| FB170 | NM_002938 | Homo sapiens ring finger protein 4 (RNF4), mRNA | TF/DNB | PFAM |
| FB291 | NM_007265 | Homo sapiens suppressor of S cerevisiae gcr2 (HSGT1), mRNA | TF/DNB | — |
| FB18 | AF060511 | Homo sapiens clone 016b10 My016 protein mRNA | TF/DNB | PFAM BoIA |
| FB183, 184 | AL832296 | EST | TF/DNB | HOX |
| FB29 | NM_015339 | Activity-dependent neuroprotective protein | TF/DNB | HOX, ZNF |
| FB225 | NM_014311 | Homo sapiens SMUG1 mRNA | TF/DNB | PFAM:UDG |
| FB2 | NM_001259 | Homo sapiens cyclin-dependent kinase 6 (CDK6) | CCR | S-TKc |
| FB91 | NM_003390 | Homo sapiens WEE1 mRNA for protein kinase | CCR | STYKc |
| FB111 | D00591 | Homo sapiens RCC1 gene | CCR | PFAM:RCC1 |
| FB25 | AL162078.1 | Homo sapiens mRNA; cDNA DKFZp761H229 | CCR | TPR |
| FB65 | AF189722 | Homo sapiens PDZ-binding kinase mRNA | CCR | STYKc |
| FB288 | NM_017943 | Homo sapiens F-box only protein 34 (FBXO34), mRNA | CCR | FBOX |
| FB251 | NM_016343 | Homo sapiens centromere protein F, 350/400ka (mitosin) (CENPF) | DNR | — |
| FB300 | AF007128 | 1-Homo sapiens clone 23870 mRNA | CCR | LRR, FBOX |
| FB128 | NM_138609 | Homo sapiens H2A histone family, member Y (H2AFY), transcript variant 1, mRNA | CRR | A1pp, H2A |
| FB100 | AF273028 | Homo sapiens NUDT10 mRNA | CRR | PFAM NUDIX |
| FB124, 240 | NM_018449 | Homo sapiens ubiquitin associated protein 2 (UBAP2), mRNA | UBQ | UBA |
| FB278 | BC019111 | Homo sapiens, sequestosome | UBQ | Contain UBA, Znf_zz and PB1 domains |
| FB145 | NM_005153 | Homo sapiens ubiquitin-specific protease 10 | DUB | PFAM |
| FB4, 33 | NM_004607 | Homo sapiens tubulin-specific chaperone a (TBCA) | PF | — |
| FB301 | NM_006788 | Homo sapiens raIA binding protein 1 (RALBP1), mRNA | ST | RhoGAP |
| FB205, 212 | AF498964 | Homo sapiens rac1 gene | ST | RHO |
| FB179, 250 | AF213884 | Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1) gene | ST | Death, ANK, PFAM:RHD, IPT |
| FB269 | AF111162 | Homo sapiens guanine nucleotide exchange factor mRNA | ST | Sec7 |
| FB48 | Y09160 | H sapiens Sub 1.5 mRNA | ST | RhoGEF, PH |
| FB114 | AF267853 | Homo sapiens HTPHLP mRNA | ST | Phosducin |
| FB44 | U50648 | Human interferon inducible RNA-dependent protein kinase | ST | DSRM, STYKc |
| FB26 | AF139768 | Homo sapiens type II transmembrane protein MDL-1 | ST | TM, CLECT |
| FB90, 96, 129, 136 | BC033731 | Homo sapiens, DIP13 beta | ST | PHD, PTB |
| FB248 | AL832326 | EST | ST | L27, GuKc, PDZ, SH3 |
| FB98 | BC030545 | EST | ST | PHD |
| FB5 | AK097395.1 | Highly similar to SUPEROXIDE DISMUTASE Homo sapiens, clone MGC | MET | PFAM |
| FB35 | AF339086 | Homo sapiens NADH dehydrogenase | MET | PFAM:oxidored |
| FB172, 181 | AY063361 | Homo sapiens isolate EICh03-98 NADH dehydrogenase subunit 4 gene | MET | PFAM:oxidored |
| FB184 | BC008906 | Homo sapiens, enoyl Coenzyme A hydratase | MET | PFAM:ECH |
| FB196 | NM_005918 | Homo sapiens malate dehydrogenase 2, NAD (mitochondrial) (MDH2) | MET | — |
| FB293 | NM_006708 | Homo sapiens glyoxalase I (GLO1), mRNA | MET | — |
| FB144 | NM_004595 | Homo sapiens spermine synthase | MET | PFAM:Spermine_synth |
| FB23, 38, 40, 226 | BC019669 | Homo sapiens, eukaryotic translation elongation factor 1 alpha 1 | PS | PFAM, GTP, EFTU, D2, D3 |
| FB171 | NM_021121 | Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 2 | PS | PFAM:EF1BD |
| FB102 | NM_006397 | Homo sapiens ribonuclease H2, large subunit (RNASEH2A), mRNA | PS | — |
| FB22, 123 | NM_000990 | Homo sapiens ribosomal protein L27a | PS | — |
| FB6, 224 | BC027620 | Homo sapiens, ribosomal protein S6 | PS | PFAM: Ribosomal_S6e |
| FB139, 140 | BC029732 | Homo sapiens, ribosomal protein S15 | PS | PFAM:Ribosomal_S15 |
| FB265 | NM_000972 | Homo sapiens ribosomal protein L7a | PS | PFAM:Ribosomal_L7Ae |
| FB125 | NM_000971 | Homo sapiens ribosomal protein L7 (RPL7), mRNA | PS | Ribosomal_L30 |
| FB42 | NM_000996 | Homo sapiens ribosomal protein L35a (RPL35A), mRNA | PS | PFAM:Ribosomal_L35Ae |
| FB28 | NM_000981 | Homo sapiens ribosomal protein L19 | PS | PFAM:Ribosomal_L19e |
| FB81 | NM_007104 | Homo sapiens ribosomal protein L10a | PS | PFAM:Ribosomal_L1 |
| FB220 | BC008003 | Homo sapiens, ribosomal protein L3 | PS | PFAM:Ribosomal_L3 |
| FB203 | BC040610 | Homo sapiens, Similar to ribosomal protein L4 | PS | — |
| FB262 | X06705 | Human PLA-X mRNA | PS | PFAM:Ribosomal_L7Ae |
| FB286 | BC007755 | Homo sapiens, glycyl-tRNA synthetase | PS | PFAM |
| FB49 | NM_001109 | Homo sapiens a disintegrin and metalloproteinase domain 8 (ADAM8) mRNA | PIP | PFAM Reprolysin, Pfam:Pep 1 DISIN, AGR, EGF, TM |
| FB30 | NM_005200 | Homo sapiens cell matrix adhesion regulator | ECM | — |
| FB20 | AL121895 | Human DNA sequence contains EPB41L1 gene encoding the erythrocyte membrane protein band | CYT | B41 |
| FB313 | BC009357 | Homo sapiens, transgelin 2 | PP | CH, PFAM:calponin |
| FB259 | NM_003883 | Histon deacetylation3 (HDAC3) | AP | PFAM-Histon Deacetyl |
| FB290 | NM_001007 | Homo sapiens ribosomal protein S4, X-linked (RPS4X), mRNA | RNB | S4, PFAM:KOW |
| FB229 | NM_005839 | Homo sapiens serine/arginine repetitive matrix 1 (SRRM1), mRNA | RNB | PW1 |
| FB243 | NM_018834 | Homo sapiens matrin 3 (MATR3), mRNA | RNB | ZnFU1, RRM |
| FB126 | NM_014252 | Homo sapiens solute carrier family 25 (mitochondrial carrier; ornithine transporter) mRNA | TRA | PFAM:mito_carr |
| FB287 | AF151028 | Homo sapiens HSPC194 mRNA | Others | PFAM 0136 |
| FB307 | NM_016424 | Homo sapiens LUC7A protein | Others | PFAM DUF 259 |
| FB200 | XM_084664 | Eythroid differentiation-related factor | Others | TM |
| FB314 | AF210651 | Homo sapiens NAG18 (NAG18) mRNA | Others | — |
| FB209 | XM_058792 | Homo sapiens similar to nuclear pore complexinteracting protein | Others | — |
| FB302 | BC031346 | Homo sapiens, clone IMAGE mRNA | Others | — |
| FB304 | AL832781 | Homo sapiens mRNA | Others | — |
| FB318 | AF225417 | Homo sapiens 88.8 kDa protein mRNA | Others | — |
| FB299 | XM_058813 | Homo sapiens similar to RIKEN cDNA | Others | — |
| FB131 | BC014508 | Homo sapiens similar to signal peptidase complex | Others | PFAM:Peptidase_S26 |
Clone . | Accession no. . | Name of gene . | Annotation . | Domain(s) . |
|---|---|---|---|---|
| FB11 | BC034041 | Homo sapiens, LIM domain only 2 | TF/DNB | LIM |
| FB271 | BC042483 | Homo sapiens, leucine zipper transcription factor-like 1 | TF/DNB | LIM |
| FB70, 305 | NM_005378 | Homo sapiens MYCN mRNA | TF/DNB | HLH, PFAM |
| FB83, 197, 245 | BC028689 | Homo sapiens transcriptional intermediary factor 1 (TIF 1), mRNA | TF/DNB | BROMO, PHD, BBC, B-BOX, RING |
| FB63 | NM_005762 | Homo sapiens tripartite motif-containing 28 | TF/DNB | BROMO, PHD, BBC, B-BOX, RING |
| FB109, 194 | J04102 | Human ETS2 mRNA | TF/DNB | ETS |
| FB170 | NM_002938 | Homo sapiens ring finger protein 4 (RNF4), mRNA | TF/DNB | PFAM |
| FB291 | NM_007265 | Homo sapiens suppressor of S cerevisiae gcr2 (HSGT1), mRNA | TF/DNB | — |
| FB18 | AF060511 | Homo sapiens clone 016b10 My016 protein mRNA | TF/DNB | PFAM BoIA |
| FB183, 184 | AL832296 | EST | TF/DNB | HOX |
| FB29 | NM_015339 | Activity-dependent neuroprotective protein | TF/DNB | HOX, ZNF |
| FB225 | NM_014311 | Homo sapiens SMUG1 mRNA | TF/DNB | PFAM:UDG |
| FB2 | NM_001259 | Homo sapiens cyclin-dependent kinase 6 (CDK6) | CCR | S-TKc |
| FB91 | NM_003390 | Homo sapiens WEE1 mRNA for protein kinase | CCR | STYKc |
| FB111 | D00591 | Homo sapiens RCC1 gene | CCR | PFAM:RCC1 |
| FB25 | AL162078.1 | Homo sapiens mRNA; cDNA DKFZp761H229 | CCR | TPR |
| FB65 | AF189722 | Homo sapiens PDZ-binding kinase mRNA | CCR | STYKc |
| FB288 | NM_017943 | Homo sapiens F-box only protein 34 (FBXO34), mRNA | CCR | FBOX |
| FB251 | NM_016343 | Homo sapiens centromere protein F, 350/400ka (mitosin) (CENPF) | DNR | — |
| FB300 | AF007128 | 1-Homo sapiens clone 23870 mRNA | CCR | LRR, FBOX |
| FB128 | NM_138609 | Homo sapiens H2A histone family, member Y (H2AFY), transcript variant 1, mRNA | CRR | A1pp, H2A |
| FB100 | AF273028 | Homo sapiens NUDT10 mRNA | CRR | PFAM NUDIX |
| FB124, 240 | NM_018449 | Homo sapiens ubiquitin associated protein 2 (UBAP2), mRNA | UBQ | UBA |
| FB278 | BC019111 | Homo sapiens, sequestosome | UBQ | Contain UBA, Znf_zz and PB1 domains |
| FB145 | NM_005153 | Homo sapiens ubiquitin-specific protease 10 | DUB | PFAM |
| FB4, 33 | NM_004607 | Homo sapiens tubulin-specific chaperone a (TBCA) | PF | — |
| FB301 | NM_006788 | Homo sapiens raIA binding protein 1 (RALBP1), mRNA | ST | RhoGAP |
| FB205, 212 | AF498964 | Homo sapiens rac1 gene | ST | RHO |
| FB179, 250 | AF213884 | Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1) gene | ST | Death, ANK, PFAM:RHD, IPT |
| FB269 | AF111162 | Homo sapiens guanine nucleotide exchange factor mRNA | ST | Sec7 |
| FB48 | Y09160 | H sapiens Sub 1.5 mRNA | ST | RhoGEF, PH |
| FB114 | AF267853 | Homo sapiens HTPHLP mRNA | ST | Phosducin |
| FB44 | U50648 | Human interferon inducible RNA-dependent protein kinase | ST | DSRM, STYKc |
| FB26 | AF139768 | Homo sapiens type II transmembrane protein MDL-1 | ST | TM, CLECT |
| FB90, 96, 129, 136 | BC033731 | Homo sapiens, DIP13 beta | ST | PHD, PTB |
| FB248 | AL832326 | EST | ST | L27, GuKc, PDZ, SH3 |
| FB98 | BC030545 | EST | ST | PHD |
| FB5 | AK097395.1 | Highly similar to SUPEROXIDE DISMUTASE Homo sapiens, clone MGC | MET | PFAM |
| FB35 | AF339086 | Homo sapiens NADH dehydrogenase | MET | PFAM:oxidored |
| FB172, 181 | AY063361 | Homo sapiens isolate EICh03-98 NADH dehydrogenase subunit 4 gene | MET | PFAM:oxidored |
| FB184 | BC008906 | Homo sapiens, enoyl Coenzyme A hydratase | MET | PFAM:ECH |
| FB196 | NM_005918 | Homo sapiens malate dehydrogenase 2, NAD (mitochondrial) (MDH2) | MET | — |
| FB293 | NM_006708 | Homo sapiens glyoxalase I (GLO1), mRNA | MET | — |
| FB144 | NM_004595 | Homo sapiens spermine synthase | MET | PFAM:Spermine_synth |
| FB23, 38, 40, 226 | BC019669 | Homo sapiens, eukaryotic translation elongation factor 1 alpha 1 | PS | PFAM, GTP, EFTU, D2, D3 |
| FB171 | NM_021121 | Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 2 | PS | PFAM:EF1BD |
| FB102 | NM_006397 | Homo sapiens ribonuclease H2, large subunit (RNASEH2A), mRNA | PS | — |
| FB22, 123 | NM_000990 | Homo sapiens ribosomal protein L27a | PS | — |
| FB6, 224 | BC027620 | Homo sapiens, ribosomal protein S6 | PS | PFAM: Ribosomal_S6e |
| FB139, 140 | BC029732 | Homo sapiens, ribosomal protein S15 | PS | PFAM:Ribosomal_S15 |
| FB265 | NM_000972 | Homo sapiens ribosomal protein L7a | PS | PFAM:Ribosomal_L7Ae |
| FB125 | NM_000971 | Homo sapiens ribosomal protein L7 (RPL7), mRNA | PS | Ribosomal_L30 |
| FB42 | NM_000996 | Homo sapiens ribosomal protein L35a (RPL35A), mRNA | PS | PFAM:Ribosomal_L35Ae |
| FB28 | NM_000981 | Homo sapiens ribosomal protein L19 | PS | PFAM:Ribosomal_L19e |
| FB81 | NM_007104 | Homo sapiens ribosomal protein L10a | PS | PFAM:Ribosomal_L1 |
| FB220 | BC008003 | Homo sapiens, ribosomal protein L3 | PS | PFAM:Ribosomal_L3 |
| FB203 | BC040610 | Homo sapiens, Similar to ribosomal protein L4 | PS | — |
| FB262 | X06705 | Human PLA-X mRNA | PS | PFAM:Ribosomal_L7Ae |
| FB286 | BC007755 | Homo sapiens, glycyl-tRNA synthetase | PS | PFAM |
| FB49 | NM_001109 | Homo sapiens a disintegrin and metalloproteinase domain 8 (ADAM8) mRNA | PIP | PFAM Reprolysin, Pfam:Pep 1 DISIN, AGR, EGF, TM |
| FB30 | NM_005200 | Homo sapiens cell matrix adhesion regulator | ECM | — |
| FB20 | AL121895 | Human DNA sequence contains EPB41L1 gene encoding the erythrocyte membrane protein band | CYT | B41 |
| FB313 | BC009357 | Homo sapiens, transgelin 2 | PP | CH, PFAM:calponin |
| FB259 | NM_003883 | Histon deacetylation3 (HDAC3) | AP | PFAM-Histon Deacetyl |
| FB290 | NM_001007 | Homo sapiens ribosomal protein S4, X-linked (RPS4X), mRNA | RNB | S4, PFAM:KOW |
| FB229 | NM_005839 | Homo sapiens serine/arginine repetitive matrix 1 (SRRM1), mRNA | RNB | PW1 |
| FB243 | NM_018834 | Homo sapiens matrin 3 (MATR3), mRNA | RNB | ZnFU1, RRM |
| FB126 | NM_014252 | Homo sapiens solute carrier family 25 (mitochondrial carrier; ornithine transporter) mRNA | TRA | PFAM:mito_carr |
| FB287 | AF151028 | Homo sapiens HSPC194 mRNA | Others | PFAM 0136 |
| FB307 | NM_016424 | Homo sapiens LUC7A protein | Others | PFAM DUF 259 |
| FB200 | XM_084664 | Eythroid differentiation-related factor | Others | TM |
| FB314 | AF210651 | Homo sapiens NAG18 (NAG18) mRNA | Others | — |
| FB209 | XM_058792 | Homo sapiens similar to nuclear pore complexinteracting protein | Others | — |
| FB302 | BC031346 | Homo sapiens, clone IMAGE mRNA | Others | — |
| FB304 | AL832781 | Homo sapiens mRNA | Others | — |
| FB318 | AF225417 | Homo sapiens 88.8 kDa protein mRNA | Others | — |
| FB299 | XM_058813 | Homo sapiens similar to RIKEN cDNA | Others | — |
| FB131 | BC014508 | Homo sapiens similar to signal peptidase complex | Others | PFAM:Peptidase_S26 |
The National Center for Biotechnology Information (NCBI) was used to identify the closest gene/transcript to the query. The SMART program was used to identify the presence of domain structure in some of the transcripts and queries. The data were annotated based on the information gained from NCBI, SMART, Gene consortium ontology, and literature search.
LIM indicates Zinc-binding domain present in Lin 11, IsI-1, and Mec-3; TF/DNB, transcription factor/DNA binding protein; HLH, helix-loop-helix; PFAM, a comprehensive database of protein domain families based on seed alignments; BROMO, bromo domain; PHD, pleckstrin homology domain; BBC, B-Box C-terminal domain; B-BOX, B-Box–type zinc finger; RING, Ring finger domain; ETS, erythroblast transformation–specific domain, http://smart.embl-heidelberg.de; BoIA, a receptor for ubiquitination targets; ZNF, zinc finger protein; UDG, uracil DNA glycosylase; CRR, chromatin regulator; S-KTc, serine threonine protein kinase; STYKc, protein kinase, unclassified specificity; TPR, tetratricopeptide repeats; F-BOX, a receptor for ubiquitination targets; DNR, DNA repair; LRR, leucine-rich repeat; A1pp,Appr-1′-p processing enzyme; H2A, histone 2A; NUDIX, http://smart.embl-heidelberg.de; UBQ, ubiquitin pathway; UBA, ubiquitin-associated; Znf_zz, zinc-binding domain present in dystrophin CREB-binding protein; PB1, PB1 domain; DUB, deubiquitination; PF, protein folding; ST, signal transduction; RhoGAP, GTPase-activator protein for Rho-like GTPases; RHO, Rho (Ras homology) subfamily of Ras-like small GTPases; ANK, ankyrin; RHD, see http://smart.embl-heidelberg.de; IPT, Ig-like, plexins, transcription factors; Sec7, Sec7 domain; RhoGEF, guanine nucleotide exchange factor for Rho/Rac/Cdc42-motif; DSRM, double-stranded RNA binding motif; TM, transmembrane domain; CLECT, C-type lectin (CTL) or carbohydrate-recognition domain (CRD); MET, metabolic genes; ECH, see http://smart.embl-heidelberg.de; PS, protein synthesis; EETU, see http://smart.embl-heidelberg.de; PIP, proteoglycan integral to plasma membrane; DISIN, homologs of snake disintegrins; AGR, see http://smart.embl-heidelberg.de; EGF, epidermal growth factor-like domain; ECM, cell adhesion and extracellular matrix; CYT, cytoskeletal; PP, protein processing; CH, calponin homology domain; AP, apoptosis; S4, S4 RNA binding domain; KOW, Kyprides Ouzonis Woese motif; PW1, PW1 domain in splicing factors; ZnFU1, U1-like zinc finger; RRM, RNA recognition motif; TRA, transporter; and –, no known domain associated with clone.