Analysis of isotype switch events and AID expression in MCL
Case . | VH status / % . | CH . | AID, wt . | AID, −ex4 . | CTs . | slg . |
|---|---|---|---|---|---|---|
| 1 | MUT/90.8 | α + γ | + | − | IαCμ | A (12%) |
| 2 | MUT/97.3 | α | + | − | − | − |
| 3 | MUT/97.6 | γ | + | − | IγCμ | − |
| 4 | MUT/96.7 | − | + | − | ND | − |
| 5 | MUT/93.8 | − | + | − | ND | − |
| 6 | MUT/94.6 | ϵ | + | − | ND | − |
| 7 | MUT/95 | − | + | + | ND | − |
| 8 | MUT/98 | α | + | + | IαCμ | − |
| 9 | UM/100 | α | + | + | IαCμ | − |
| 10 | UM/100 | α | + | + | − | − |
| 11 | UM/100 | γ | + | + | IγCμ | − |
| 12* | UM/99.3 | − | − | + | ND | − |
| 13 | UM/100 | − | − | − | ND | ND |
| 14 | UM/98.6 | ND | + | + | ND | ND |
| 15 | UM/100 | ND | + | + | ND | ND |
| 16 | UM/98.3 | ND | + | + | ND | ND |
| 17 | UM/98.6 | ND | + | + | ND | ND |
| 18 | UM/98.3 | ND | + | + | ND | ND |
Case . | VH status / % . | CH . | AID, wt . | AID, −ex4 . | CTs . | slg . |
|---|---|---|---|---|---|---|
| 1 | MUT/90.8 | α + γ | + | − | IαCμ | A (12%) |
| 2 | MUT/97.3 | α | + | − | − | − |
| 3 | MUT/97.6 | γ | + | − | IγCμ | − |
| 4 | MUT/96.7 | − | + | − | ND | − |
| 5 | MUT/93.8 | − | + | − | ND | − |
| 6 | MUT/94.6 | ϵ | + | − | ND | − |
| 7 | MUT/95 | − | + | + | ND | − |
| 8 | MUT/98 | α | + | + | IαCμ | − |
| 9 | UM/100 | α | + | + | IαCμ | − |
| 10 | UM/100 | α | + | + | − | − |
| 11 | UM/100 | γ | + | + | IγCμ | − |
| 12* | UM/99.3 | − | − | + | ND | − |
| 13 | UM/100 | − | − | − | ND | ND |
| 14 | UM/98.6 | ND | + | + | ND | ND |
| 15 | UM/100 | ND | + | + | ND | ND |
| 16 | UM/98.3 | ND | + | + | ND | ND |
| 17 | UM/98.6 | ND | + | + | ND | ND |
| 18 | UM/98.3 | ND | + | + | ND | ND |
Circle transcripts (CTs) were analyzed as described,15 with sequence analysis confirming identity of RT-PCR products at the nucleotide level (data not shown). CT sequences derived from the MCL cases have been deposited in the EMBL databases (AJ617576-AJ617579). For Iγ-Cμ, CTs were generated from alternative splice sites in Sγ. As controls for CT analysis, 2 CLL cases with unmutated VH genes were analyzed under identical conditions,15 and found positive for Iγ-Cμ/Iγ-Cμ (data not shown).
+ indicates present; −, absent; and ND, not determined.
In case no. 12, a role for AID lacking exon 4 in class switch recombination is unclear.