Effect of platelet inhibitors on LPA-and ADP-induced platelet aggregation in whole blood
. | Platelet aggregation, % . | . | . | ||
|---|---|---|---|---|---|
. | Control . | LPA, 20 μM . | ADP, 5 μM . | ||
| Buffer | 18 ± 12 (6) | 72 ± 26 (6) | 89 ± 18 (6) | ||
| Aspirin | — | 72 ± 13 (5) | — | ||
| Apyrase | — | 17 ± 11 (6)* | — | ||
| AR-C69931MX | — | 7 ± 3 (3)† | 15 ± 12 (6)* | ||
| MRS2179 | — | 9 ± 5 (3)† | 28 ± 16 (6)* | ||
| AR-C69931MX + MRS2179 | — | 10 ± 4 (6)† | 13 ± 6 (6)† | ||
| RGDS | — | 19 ± 9 (6)* | — | ||
| Anti-P-selectin | — | 61 ± 14 (4) | — | ||
. | Platelet aggregation, % . | . | . | ||
|---|---|---|---|---|---|
. | Control . | LPA, 20 μM . | ADP, 5 μM . | ||
| Buffer | 18 ± 12 (6) | 72 ± 26 (6) | 89 ± 18 (6) | ||
| Aspirin | — | 72 ± 13 (5) | — | ||
| Apyrase | — | 17 ± 11 (6)* | — | ||
| AR-C69931MX | — | 7 ± 3 (3)† | 15 ± 12 (6)* | ||
| MRS2179 | — | 9 ± 5 (3)† | 28 ± 16 (6)* | ||
| AR-C69931MX + MRS2179 | — | 10 ± 4 (6)† | 13 ± 6 (6)† | ||
| RGDS | — | 19 ± 9 (6)* | — | ||
| Anti-P-selectin | — | 61 ± 14 (4) | — | ||
Values are mean ± SD (n). The percentage aggregation was calculated as percentage loss of single platelets compared to baseline count. Concentrations of platelet inhibitors were: aspirin, 1 mM; AR-C69931MX, 1 μM; MRS2179, 100 μM; RGDS, 5 mM; apyrase 10 U/mL; and anti-P-selectin antibody 10 μg/mL. LPA was 1-acyl-LPA (16:0). — indicates no experiments have been done.
P < .05
P < .001