Specificity, isotype, source, and properties of mAbs used
. | . | . | . | Redistribution to rafts . | . | |
|---|---|---|---|---|---|---|
| mAb . | Specificity . | Isotype . | Source . | No x-link . | + x-link . | |
| OKT3 | CD3 | mlgG2a | ATCC Hybridoma | − | − | |
| IF5 | CD20 | mlgG2a | ECACC Hybridoma | ++ | ++++ | |
| B1 | CD20 | mlgG2a | Coulter, Miami, FL | −/+ | + | |
| Rituximab | CD20 | Chimeric Hu Fc | IDEC, San Francisco, CA | +++ | ++++ | |
| WR17 | CD37 | mlgG2a | Moore et al15 | − | +++ | |
| 1B4 | CD38 | mlgG2a | Funaro et al16 | − | +++ | |
. | . | . | . | Redistribution to rafts . | . | |
|---|---|---|---|---|---|---|
| mAb . | Specificity . | Isotype . | Source . | No x-link . | + x-link . | |
| OKT3 | CD3 | mlgG2a | ATCC Hybridoma | − | − | |
| IF5 | CD20 | mlgG2a | ECACC Hybridoma | ++ | ++++ | |
| B1 | CD20 | mlgG2a | Coulter, Miami, FL | −/+ | + | |
| Rituximab | CD20 | Chimeric Hu Fc | IDEC, San Francisco, CA | +++ | ++++ | |
| WR17 | CD37 | mlgG2a | Moore et al15 | − | +++ | |
| 1B4 | CD38 | mlgG2a | Funaro et al16 | − | +++ | |
In the isotype column, m indicates mouse and Hu indicates human immunoglobulins. Raft redistribution in the presence (+ x-link) or absence (no x-link) of hyper-cross-linking agent was determined by insolubility in 0.5% TX-100 as detailed previously.14 The number of +'s signify the extent of redistribution.