Vδ2-Jα rearrangements in normal lymphoid tissues as compared with precursor-B—ALL
. | Vδ2-Jα61 . | Vδ2-Jα58 . | Vδ2-Jα29 . | Vδ2-Jα9 . |
|---|---|---|---|---|
| Precursor-B—ALL, % of Vδ2-Jα-positive cases | 4.4 | 3.8 | 53.8 | 6.3 |
| CD19+/CD10++/CD20- sorted normal BM B-cell precursors; n = 1*† | - | - | - | - |
| CD19+/CD10+/CD20+/- sorted normal BM B-cell precursors; n = 1† | - | - | ± | - |
| CD19+/CD10-/CD20+ sorted normal BM B cells; n = 1† | - | - | - | - |
| Regenerating BM; n = 3‡ | ± | - | - | - |
| Normal BM; n = 3 | ±/+ | ± | - | - |
| Normal PB; n = 2 | + | ± | - | - |
| E-rosette-positive PB MNCs; n = 1 | + | ±/+ | - | - |
| E-rosette-negative PB MNCs; n = 1 | - | - | - | - |
| Tonsil; n = 2 | ± | ± | ± | - |
| Lymph node; n = 3 | ± | ±/+ | ± | - |
| Thymus; n = 3 | ++ | +/++ | ± | - |
. | Vδ2-Jα61 . | Vδ2-Jα58 . | Vδ2-Jα29 . | Vδ2-Jα9 . |
|---|---|---|---|---|
| Precursor-B—ALL, % of Vδ2-Jα-positive cases | 4.4 | 3.8 | 53.8 | 6.3 |
| CD19+/CD10++/CD20- sorted normal BM B-cell precursors; n = 1*† | - | - | - | - |
| CD19+/CD10+/CD20+/- sorted normal BM B-cell precursors; n = 1† | - | - | ± | - |
| CD19+/CD10-/CD20+ sorted normal BM B cells; n = 1† | - | - | - | - |
| Regenerating BM; n = 3‡ | ± | - | - | - |
| Normal BM; n = 3 | ±/+ | ± | - | - |
| Normal PB; n = 2 | + | ± | - | - |
| E-rosette-positive PB MNCs; n = 1 | + | ±/+ | - | - |
| E-rosette-negative PB MNCs; n = 1 | - | - | - | - |
| Tonsil; n = 2 | ± | ± | ± | - |
| Lymph node; n = 3 | ± | ±/+ | ± | - |
| Thymus; n = 3 | ++ | +/++ | ± | - |
Vδ2-Jα rearrangements were quantified by RQ-PCR, and the obtained values were grouped into the following categories: ++, Vδ2-Jα levels more than 10-3; +, Vδ2-Jα levels between 10-4 and 10-3; ±, Vδ2-Jα levels between 10-5 and 10-4; -, Vδ2-Jα levels 10-5 or less or undetectable.
Numbers correspond to number of samples tested.
Normal BM was stained with CD10, CD20, and CD19 monoclonal antibodies, and the indicated cell populations were sorted using a Diva flow cytometer (BD Biosciences, San Jose, CA).
Regenerating BM after cessation of chemotherapy is known to contain high frequencies of precursor-B cells (5% to 30%).30,31