Reduced levels but normal lineage distribution of CFCs in murine BM cells expressing mut10-C/EBPα
. | No. of colonies per 104 cells . | Mean distribution of mature colonies, % . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Vector . | . | CFU-Mix . | CFU-GM . | CFU-G . | CFU-M . | BFU-E . | ||||
| FMEV-mut10 | 39 ± 7 | 2.5 ± 1.4 | 60 ± 9.7 | 2.5 ± 0.4 | 28 ± 15.2 | 7.5 ± 5.4 | ||||
| FMEV-GFP | 88 ± 18 | 2.5 ± 1.4 | 65 ± 1.7 | 5.1 ± 2.8 | 23 ± 1.9 | 3.8 ± 1.6 | ||||
| Mock infected | 95 ± 6 | 1.0 ± 1 | 53 ± 1.2 | 4.1 ± 1.2 | 29 ± 7.3 | 13 ± 7.7 | ||||
. | No. of colonies per 104 cells . | Mean distribution of mature colonies, % . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Vector . | . | CFU-Mix . | CFU-GM . | CFU-G . | CFU-M . | BFU-E . | ||||
| FMEV-mut10 | 39 ± 7 | 2.5 ± 1.4 | 60 ± 9.7 | 2.5 ± 0.4 | 28 ± 15.2 | 7.5 ± 5.4 | ||||
| FMEV-GFP | 88 ± 18 | 2.5 ± 1.4 | 65 ± 1.7 | 5.1 ± 2.8 | 23 ± 1.9 | 3.8 ± 1.6 | ||||
| Mock infected | 95 ± 6 | 1.0 ± 1 | 53 ± 1.2 | 4.1 ± 1.2 | 29 ± 7.3 | 13 ± 7.7 | ||||
Mock-infected or vector-infected Linneg BM cells sorted for GFP expression were plated in methylcellulose at 2 different cell concentrations and scored for colony formation and morphology after 7 days. Results shown are from one experiment performed in triplicate. Similar values were obtained in a second independent experiment. CFU-Mix indicates mixed colony-forming unit; CFU-GM, granulocyte macrophage colony-forming unit; and BFU-E, erythroid burst-forming unit.