Human CD34+ CB cells expressing mut10-C/EBPα do not give rise to normal colonies
. | No. of colonies per 103 cells . | Mean distribution of colonies, % . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Vector . | . | CFU-GEMM . | CFU-GM . | CFU-G . | CFU-M . | BFU-E . | ||||
| FMEV-mut10 | 154 ± 14 | 0 | 0 | 0 | 0 | 0.3 ± 0.2 | ||||
| FMEV-GFP | 149 ± 20 | 2.2 ± 1.6 | 6.3 ± 0.4 | 14 ± 8 | 29.5 ± 12 | 48 ± 15 | ||||
. | No. of colonies per 103 cells . | Mean distribution of colonies, % . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Vector . | . | CFU-GEMM . | CFU-GM . | CFU-G . | CFU-M . | BFU-E . | ||||
| FMEV-mut10 | 154 ± 14 | 0 | 0 | 0 | 0 | 0.3 ± 0.2 | ||||
| FMEV-GFP | 149 ± 20 | 2.2 ± 1.6 | 6.3 ± 0.4 | 14 ± 8 | 29.5 ± 12 | 48 ± 15 | ||||
Four days after isolation of CD34+ CB cells, mock-infected, or vector-infected cells sorted for GFP expression were plated in methylcellulose at 2 different cell concentrations and scored for colony formation and morphology after 11 days. Results are the average of 2 independent experiments, each performed in triplicate. CFU-GEMM indicates granulocyte-erythrocyte-megakaryocyte-macrophage colony-forming unit.