Primary antibodies used in immunofluorescence double staining
Staining . | Primary antibody . | Source . |
|---|---|---|
| tPA/microglia | RabtPA | American Diagnostica, Greenwich, CT |
| Rat antimouse F4/80 | Serotec, Oxford, United Kingdom | |
| tPA/astrocyte | RabtPA | American Diagnostica |
| GFAP | Zymed Laboratories, South San Francisco, CA | |
| tPA/neuron | GoatPA | Santa Cruz Biotechnology, Santa Cruz, CA |
| NEURO | Chemicon, Temecula, CA | |
| tPA/endothelial | GoatPA | Santa Cruz Biotechnology |
| Polyclonal rabbit antihuman VWF | DAKO, Carpinteria, CA | |
| MMP 9/endothelial | Goat antimouse MMP 9 | R&D Systems, Minneapolis, MN |
| Polyclonal rabbit antihuman VWF | DAKO | |
| MMP 9/microglia | Goat antimouse MMP 9 | R&D Systems |
| Rat antimouse F4/80 | Serotec |
Staining . | Primary antibody . | Source . |
|---|---|---|
| tPA/microglia | RabtPA | American Diagnostica, Greenwich, CT |
| Rat antimouse F4/80 | Serotec, Oxford, United Kingdom | |
| tPA/astrocyte | RabtPA | American Diagnostica |
| GFAP | Zymed Laboratories, South San Francisco, CA | |
| tPA/neuron | GoatPA | Santa Cruz Biotechnology, Santa Cruz, CA |
| NEURO | Chemicon, Temecula, CA | |
| tPA/endothelial | GoatPA | Santa Cruz Biotechnology |
| Polyclonal rabbit antihuman VWF | DAKO, Carpinteria, CA | |
| MMP 9/endothelial | Goat antimouse MMP 9 | R&D Systems, Minneapolis, MN |
| Polyclonal rabbit antihuman VWF | DAKO | |
| MMP 9/microglia | Goat antimouse MMP 9 | R&D Systems |
| Rat antimouse F4/80 | Serotec |