Description of the fiber-FISH results for patients and cell lines
Patients and HMCLs . | Isotype . | Interphase FISH results . | Fiber-FISH results for first allele*[AU8] . | Fiber-FISH results for second allele* . | Fiber-FISH results for third allele . |
|---|---|---|---|---|---|
| OPM2 HMCL | LC λ | t(4;14) | FGFR3-γ2-γ4-ϵ-α2 | γ1-α1 (inserted at 8q24) | — |
| MDN HMCL | LC κ | t(11;14) | CCND1?-ψγ-γ2-γ4-ϵ-α2 | CCND1?-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| XG5 HMCL | LC λ | t(11;14) | CCND1 (799B16)-μ?-γ2-γ4-ϵ-α2 | μ-γ3-γ1-ψγ-γ2-γ4-ϵ-α2 | — |
| SKMM1 HMCL | LC κ | t(14;20) | MAFB-ψγ-γ2-γ4-ϵ-α2 | μ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | μ-ψγ-γ2-γ4-ϵ-α2 |
| ANBL6 HMCL | LC λ | t(14;16) | MAF?-μ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | MAF?-μ-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 1 | LC λ | t(11;14) | CCND1 (799B16)-γ2-γ4-ϵ-α2 | α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 2 | LC λ | t(11;14) | CCND1?-α1-ψγ-γ2-γ4-ϵ-α2 | CCND1?-μ-γ2-γ4-ϵ-α2 | — |
| Patient 3 | LC κ | t(4;14) | FGFR3-μ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1 -α1-ψγ-γ-γ2-γ4-ϵ-α2 | — |
| Patient 4 | LC λ | t(11;14) | CCND1?-μ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | CCND1?-D-J-μ-δ-γ3-γ1 -α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 5 | LC κ | t(11;14) | CCND1 (799B16)-D-J-μ-δ-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1 -ψγ-γ-γ2-γ4-ϵ-α2 | — |
| Patient 6 | LC κ | R | DJ-μ-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 7 | LC λ | R | D-J-μ-δ-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 8 | LC κ | R | D-J-μ-δ-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1 -ψγ-γ-γ2-γ4-ϵ-α2 | — |
| Patient 9 | LC κ | R | μ-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1 -ψγ-γ-γ2-γ4-ϵ-α2 | — |
| Patient 10 | LC λ | G | D-J-μ-δ-γ3-γ1-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 11 | LC κ | G | D-J-μ-δ-γ3-γ1-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| U266 HMCL | IgE | t(11;14) | μ-ϵ | CCND1 (6-22)-α1-ψγ-γ2-γ4 | — |
| LP1 HMCL | IgGλ | t(4;14) | μ-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | FGFR3-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 12 | IgGκ | t(11;14) | μ-γ4-ϵ-α2 | CCND1?-γ-γ2-γ4-ϵ-α2 | — |
| Patient 13 | IgGκ | t(4;14) | μ-γ2-γ4-ϵ-α2 | FGFR3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 14 | IgAκ | t(4;14) | μ-α2 | FGFR3-γ2-γ4-ϵ-α2 | — |
| Patient 15 | IgAλ | R | μ-α1-ψγ-γ-γ2-γ4-ϵ-α2 | ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 16 | IgGλ | R | μ-γ1-ψγ-γ-γ2-γ4-ϵ-α2 | γ2-γ4-ϵ-α2 | — |
| Patient 17 | NS | t(11;14) | D-J-ψγ-γ2-γ4-ϵ-α2 | CCND1 (799B16)-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
Patients and HMCLs . | Isotype . | Interphase FISH results . | Fiber-FISH results for first allele*[AU8] . | Fiber-FISH results for second allele* . | Fiber-FISH results for third allele . |
|---|---|---|---|---|---|
| OPM2 HMCL | LC λ | t(4;14) | FGFR3-γ2-γ4-ϵ-α2 | γ1-α1 (inserted at 8q24) | — |
| MDN HMCL | LC κ | t(11;14) | CCND1?-ψγ-γ2-γ4-ϵ-α2 | CCND1?-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| XG5 HMCL | LC λ | t(11;14) | CCND1 (799B16)-μ?-γ2-γ4-ϵ-α2 | μ-γ3-γ1-ψγ-γ2-γ4-ϵ-α2 | — |
| SKMM1 HMCL | LC κ | t(14;20) | MAFB-ψγ-γ2-γ4-ϵ-α2 | μ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | μ-ψγ-γ2-γ4-ϵ-α2 |
| ANBL6 HMCL | LC λ | t(14;16) | MAF?-μ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | MAF?-μ-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 1 | LC λ | t(11;14) | CCND1 (799B16)-γ2-γ4-ϵ-α2 | α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 2 | LC λ | t(11;14) | CCND1?-α1-ψγ-γ2-γ4-ϵ-α2 | CCND1?-μ-γ2-γ4-ϵ-α2 | — |
| Patient 3 | LC κ | t(4;14) | FGFR3-μ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1 -α1-ψγ-γ-γ2-γ4-ϵ-α2 | — |
| Patient 4 | LC λ | t(11;14) | CCND1?-μ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | CCND1?-D-J-μ-δ-γ3-γ1 -α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 5 | LC κ | t(11;14) | CCND1 (799B16)-D-J-μ-δ-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1 -ψγ-γ-γ2-γ4-ϵ-α2 | — |
| Patient 6 | LC κ | R | DJ-μ-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 7 | LC λ | R | D-J-μ-δ-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 8 | LC κ | R | D-J-μ-δ-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1 -ψγ-γ-γ2-γ4-ϵ-α2 | — |
| Patient 9 | LC κ | R | μ-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1 -ψγ-γ-γ2-γ4-ϵ-α2 | — |
| Patient 10 | LC λ | G | D-J-μ-δ-γ3-γ1-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 11 | LC κ | G | D-J-μ-δ-γ3-γ1-ψγ-γ2-γ4-ϵ-α2 | D-J-μ-δ-γ3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| U266 HMCL | IgE | t(11;14) | μ-ϵ | CCND1 (6-22)-α1-ψγ-γ2-γ4 | — |
| LP1 HMCL | IgGλ | t(4;14) | μ-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | FGFR3-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 12 | IgGκ | t(11;14) | μ-γ4-ϵ-α2 | CCND1?-γ-γ2-γ4-ϵ-α2 | — |
| Patient 13 | IgGκ | t(4;14) | μ-γ2-γ4-ϵ-α2 | FGFR3-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 14 | IgAκ | t(4;14) | μ-α2 | FGFR3-γ2-γ4-ϵ-α2 | — |
| Patient 15 | IgAλ | R | μ-α1-ψγ-γ-γ2-γ4-ϵ-α2 | ψγ-γ2-γ4-ϵ-α2 | — |
| Patient 16 | IgGλ | R | μ-γ1-ψγ-γ-γ2-γ4-ϵ-α2 | γ2-γ4-ϵ-α2 | — |
| Patient 17 | NS | t(11;14) | D-J-ψγ-γ2-γ4-ϵ-α2 | CCND1 (799B16)-γ1-α1-ψγ-γ2-γ4-ϵ-α2 | — |
R indicates patients with an illegitimately rearranged IgH allele, but with an unidentified chromosomal partner; G, no illegitimate IgH rearrangement was observed using interphase FISH; and —, no results.
The succession of the constant domains is described. When the colocalization of the IgH probes and the chromosomal partner probe was not observed (because of a too large physical distance between them), a “?” was added after the probe name. When the U2-2 and the 3/64 probes were not rearranged (signing a germline configuration of the D and J regions), the domains were written “D-J.” In contrast, when a rearrangement occurred, it was written “DJ.” CCND1 represents the 11q13 region, MAF represents the 16q23 region, and MAFB represents the 20q11 region.