HSC frequency and distribution in different subsets of bone marrow cells from adult mice given 5-FU 4 days previously
Phenotype (% of total*) and no. cells per mouse . | No. positive mice/total no. mice . | HSC frequency†(upper and lower limits) . | % of HSCs in fraction‡ . |
|---|---|---|---|
| SP Rho−(0.22 ± 0.06) | 1/37 (1/21-1/65) | 27 | |
| 39 | 2/2 | ||
| 12 | 0/5 | ||
| 6 | 0/2 | ||
| 4 | 1/2 | ||
| SP Rho+(0.55 ± 0.004) | 1/37 (1/24-1/56) | 67 | |
| 64 | 3/3 | ||
| 30 | 3/6 | ||
| 7 | 0/3 | ||
| Non-SP Rho−(8.2 ± 1.9) | < 1/15 000¶ (< 1/5600-< 1/38 000) | <2¶ | |
| 11 000 | 0/1 | ||
| 4500 | 0/2 | ||
| 500 | 0/2 | ||
| Non-SP Rho+(84 ± 5) | 1/91 000 (1/45 000-1/180 000) | 4 | |
| 150 000 | 2/2 | ||
| 22 000 | 0/3 | ||
| 1900 | 0/4 |
Phenotype (% of total*) and no. cells per mouse . | No. positive mice/total no. mice . | HSC frequency†(upper and lower limits) . | % of HSCs in fraction‡ . |
|---|---|---|---|
| SP Rho−(0.22 ± 0.06) | 1/37 (1/21-1/65) | 27 | |
| 39 | 2/2 | ||
| 12 | 0/5 | ||
| 6 | 0/2 | ||
| 4 | 1/2 | ||
| SP Rho+(0.55 ± 0.004) | 1/37 (1/24-1/56) | 67 | |
| 64 | 3/3 | ||
| 30 | 3/6 | ||
| 7 | 0/3 | ||
| Non-SP Rho−(8.2 ± 1.9) | < 1/15 000¶ (< 1/5600-< 1/38 000) | <2¶ | |
| 11 000 | 0/1 | ||
| 4500 | 0/2 | ||
| 500 | 0/2 | ||
| Non-SP Rho+(84 ± 5) | 1/91 000 (1/45 000-1/180 000) | 4 | |
| 150 000 | 2/2 | ||
| 22 000 | 0/3 | ||
| 1900 | 0/4 |
Values represent the mean ± SEM from 2 experiments.
HSC frequencies were calculated with data obtained more than 4 months after transplantation. Values shown in parentheses are the upper and lower limits defined by ± SEM. Levels of PB cells derived from the test transplants in the positive recipients at the time of analysis varied from 4% to 65%.
Calculated as described in Table 1.
Calculated assuming one mouse given the maximum number of cells tested had been positive for donor cells.