HSC frequency and distribution in different subsets of cultured Sca-1 + lin−adult bone marrow cells
Phenotype (% of total*) and no. cells per mouse . | No. positive mice/total no. mice . | HSC frequency†(upper and lower limits) . | % of HSCs in fraction‡ . |
|---|---|---|---|
| Lin−SP Rho−(0.005 ± 0.002) | < 1/130¶ (< 1/50-< 1/340) | < 6¶ | |
| 77 | 0/1 | ||
| 36 | 0/2 | ||
| 12 | 0/2 | ||
| Lin−SP Rho+(0.075 ± 0.025) | 1/1000 (< 1/600-< 1/1800) | 12 | |
| 1500 | 2/2 | ||
| 580 | 1/2 | ||
| 550 | 0/2 | ||
| Lin−non-SP Rho−(4.0 ± 1.1) | < 1/35 000¶ (< 1/5000-< 1/93 000) | < 19¶ | |
| 19 000 | 0/2 | ||
| 2100 | 0/2 | ||
| 1400 | 0/2 | ||
| Lin−non-SP Rho+(75 ± 3) | 1/200 000 (< 1/130 000-< 1/310 000) | 63 | |
| 60 000 | 5/19 |
Phenotype (% of total*) and no. cells per mouse . | No. positive mice/total no. mice . | HSC frequency†(upper and lower limits) . | % of HSCs in fraction‡ . |
|---|---|---|---|
| Lin−SP Rho−(0.005 ± 0.002) | < 1/130¶ (< 1/50-< 1/340) | < 6¶ | |
| 77 | 0/1 | ||
| 36 | 0/2 | ||
| 12 | 0/2 | ||
| Lin−SP Rho+(0.075 ± 0.025) | 1/1000 (< 1/600-< 1/1800) | 12 | |
| 1500 | 2/2 | ||
| 580 | 1/2 | ||
| 550 | 0/2 | ||
| Lin−non-SP Rho−(4.0 ± 1.1) | < 1/35 000¶ (< 1/5000-< 1/93 000) | < 19¶ | |
| 19 000 | 0/2 | ||
| 2100 | 0/2 | ||
| 1400 | 0/2 | ||
| Lin−non-SP Rho+(75 ± 3) | 1/200 000 (< 1/130 000-< 1/310 000) | 63 | |
| 60 000 | 5/19 |
Values represent the mean ± SEM from 3 experiments.
HSC frequencies were calculated with data obtained 4 months after transplantation. Values shown in parentheses are the upper and lower limits defined by ± SEM. Levels of PB cells derived from the test transplants in the positive recipients at the time of analysis varied from 1% to 18%.
Calculated as described in Table 1 for the HSCs in each lin− fraction analyzed that had been derived from 5 × 105 starting Sca-1+ adult bone marrow cells.
Calculated assuming one mouse given the maximum number of cells tested had been positive for donor cells