Table 1. Specificity constants of... | American Society of Hematology

Table 1.

Specificity constants of thrombin-catalyzed fibrinopeptide release in the presence and the absence of added CaCl₂

	No added CaCl₂, k_cat/K_m ± SD			1 mM CaCl₂, k_cat/K_m ± SD
Fibrinogen	FpA	FpB (P)	FpA (P)	FpB (P)
Normal	13.6 ± 3.8	5.9 ± 1.4	10.9 ± 1.4 (.3768^*)	6.8 ± 0.3 (.3091^*)
γN308K	11.9 ± 2.1	2.2 ± 0.3 (.0027†)	11.4 ± 0.7 (.1231^*)	2.9 ± 0.2 (.0015†; .0634^*)
γN308A	11.6 ± 2.0	4.9 ± 1.8 (.0474†)	11.2 ± 1.1 (.1732^*)	8.6 ± 0.4 (.0159†; .0530^*)
γN308I	11.6 ± 0.4	3.0 ± 1.0 (.0097†)	11.3 ± 0.5 (.1276^*)	7.2 ± 0.3 (.1091†; .0189^*)

	No added CaCl₂, k_cat/K_m ± SD			1 mM CaCl₂, k_cat/K_m ± SD
Fibrinogen	FpA	FpB (P)	FpA (P)	FpB (P)
Normal	13.6 ± 3.8	5.9 ± 1.4	10.9 ± 1.4 (.3768^*)	6.8 ± 0.3 (.3091^*)
γN308K	11.9 ± 2.1	2.2 ± 0.3 (.0027†)	11.4 ± 0.7 (.1231^*)	2.9 ± 0.2 (.0015†; .0634^*)
γN308A	11.6 ± 2.0	4.9 ± 1.8 (.0474†)	11.2 ± 1.1 (.1732^*)	8.6 ± 0.4 (.0159†; .0530^*)
γN308I	11.6 ± 0.4	3.0 ± 1.0 (.0097†)	11.3 ± 0.5 (.1276^*)	7.2 ± 0.3 (.1091†; .0189^*)

All units are 10⁶ M^-1S^-1 ± SD. The k_cat/K_m values were calculated as described in Ng et al³⁴ and Lord et al.³⁵

P values of an unpaired t test comparing the addition of 0 or 1 mM CaCl₂

P values of an unpaired t test to compare a variant to normal fibrinogen