List of genes preferentially expressed in HEVECs that exhibit rapid down-regulation ex vivo
GenBank no. . | Gene name . | First screen D0 HEVECs/D0 HUVECs . | Second screen D0 HEVECs/D2 HEVECs . |
|---|---|---|---|
| Cell signaling and extracellular communication proteins | |||
| M86528 | Neurotrophin-4 (NT-4) | 100 | 13 |
| X02812, J05114 | Transforming growth factor-β (TGF-β) | 44 | 5.4 |
| M96955, M96956 | Teratocarcinoma-derived growth factor 1 (TDGF1) | 24 | 4.8 |
| Extracellular matrix proteins | |||
| L25286 | Collagen 15α1 subunit (COL 15A1) | 28 | 5.2 |
| Cytokine and chemokine receptors | |||
| X68203, X69878 | VEGFR-3 | ND | 5.9 |
| M27492 | Interleukin-1 receptor type 1 precursor (IL-1R1) | 33 | 18 |
| U01839 | Duffy blood group antigen DARC | 8.9 | 430 |
| Cell adhesion receptors and proteins | |||
| L12002, X16983 | Integrin α4 precursor (ITGA4) | ND | 31 |
| M30640 | E-selectin (SELE), CD62E antigen | 80 | 5.0 |
| Membrane channels and transporters | |||
| M15800 | MAL | 69 | 4.3 |
| U77086 | Organic cation transporter 1 | 6.0 | 7.0 |
| Intracellular transducers, effectors, and modulators | |||
| U70426, AF009356 | Regulator of G-protein signaling 16/A28-RGS14p | ND | 230 |
| M86757 | S100 calcium-binding protein A7; psoriasin | 53 | 14 |
| L41816 | Calcium/calmodulin-dependent protein kinase I | 30 | 8.2 |
| U18087 | 3′5′-cyclic adenosine monophosphate (cAMP) phosphodiesterase HPDE4A6 | 19 | 5.5 |
| Transcription- and DNA-binding factors | |||
| X66087 | A-myb proto-oncogene; myb-related protein A | 320 | 53 |
| M32865, S38729 | Ku 70-kDa subunit | 41 | 12 |
| U73328 | Homeobox protein DLX-4 (DLX-7) (DLX-8) | 9.8 | 13 |
| Apoptosis-associated proteins | |||
| U66879 | BAD protein | 38 | 7.5 |
| L07414 | CD40 ligand (CD40-L) | 32 | 8.9 |
| Others | |||
| J02871 | Cytochrome P450 IVB1 (P450-HP) | ND | 8.6 |
| M10321 | VWF | 9.9 | 53 |
| X87212 | Dipeptidyl-peptidase I (DPP-I), cathepsin C, cathepsin J, dipeptidyl transferase | 7.5 | 8.5 |
GenBank no. . | Gene name . | First screen D0 HEVECs/D0 HUVECs . | Second screen D0 HEVECs/D2 HEVECs . |
|---|---|---|---|
| Cell signaling and extracellular communication proteins | |||
| M86528 | Neurotrophin-4 (NT-4) | 100 | 13 |
| X02812, J05114 | Transforming growth factor-β (TGF-β) | 44 | 5.4 |
| M96955, M96956 | Teratocarcinoma-derived growth factor 1 (TDGF1) | 24 | 4.8 |
| Extracellular matrix proteins | |||
| L25286 | Collagen 15α1 subunit (COL 15A1) | 28 | 5.2 |
| Cytokine and chemokine receptors | |||
| X68203, X69878 | VEGFR-3 | ND | 5.9 |
| M27492 | Interleukin-1 receptor type 1 precursor (IL-1R1) | 33 | 18 |
| U01839 | Duffy blood group antigen DARC | 8.9 | 430 |
| Cell adhesion receptors and proteins | |||
| L12002, X16983 | Integrin α4 precursor (ITGA4) | ND | 31 |
| M30640 | E-selectin (SELE), CD62E antigen | 80 | 5.0 |
| Membrane channels and transporters | |||
| M15800 | MAL | 69 | 4.3 |
| U77086 | Organic cation transporter 1 | 6.0 | 7.0 |
| Intracellular transducers, effectors, and modulators | |||
| U70426, AF009356 | Regulator of G-protein signaling 16/A28-RGS14p | ND | 230 |
| M86757 | S100 calcium-binding protein A7; psoriasin | 53 | 14 |
| L41816 | Calcium/calmodulin-dependent protein kinase I | 30 | 8.2 |
| U18087 | 3′5′-cyclic adenosine monophosphate (cAMP) phosphodiesterase HPDE4A6 | 19 | 5.5 |
| Transcription- and DNA-binding factors | |||
| X66087 | A-myb proto-oncogene; myb-related protein A | 320 | 53 |
| M32865, S38729 | Ku 70-kDa subunit | 41 | 12 |
| U73328 | Homeobox protein DLX-4 (DLX-7) (DLX-8) | 9.8 | 13 |
| Apoptosis-associated proteins | |||
| U66879 | BAD protein | 38 | 7.5 |
| L07414 | CD40 ligand (CD40-L) | 32 | 8.9 |
| Others | |||
| J02871 | Cytochrome P450 IVB1 (P450-HP) | ND | 8.6 |
| M10321 | VWF | 9.9 | 53 |
| X87212 | Dipeptidyl-peptidase I (DPP-I), cathepsin C, cathepsin J, dipeptidyl transferase | 7.5 | 8.5 |
Genes preferentially expressed in HEVECs were identified by comparing normalized hybridization signals between D0 HEVEC and D HUVEC samples (first screen); genes down-regulated in dedifferentiated HEVECs were found by comparing D0 HEVEC and D2 HEVEC samples (second screen). Only genes preferentially expressed in D0 HEVECs in the 2 screens (ratio greater than 4) are included in Table 1. Results shown are derived from single representative D0 HEVEC and D2 HEVEC samples. However, similar results were obtained with independent D0 HEVEC and D2 HEVEC samples. ND indicates not detected in HUVEC sample.