Genes significantly overexpressed or underexpressed in HS-27a/CD14+ monocyte cocultures compared with either population cultured alone
Clone ID . | Accession . | Cluster ID . | Name . | Annotation . | Ratio* . | P† . |
|---|---|---|---|---|---|---|
| 378461 | AA775616 | Hs.313 | SPP1 | Osteopontin | 15.4 | 1.63 × 10−6 |
| 418422 | W92812 | Hs.2164 | PPBP | CXCL7 | 13.7 | 2.69 × 10−9 |
| 840493 | AA485893 | Hs.78224 | RNASE1 | Ribonuclease, RNase A family | 7.6 | 4.36 × 10−8 |
| 773330 | AA425450 | — | — | ESTs | 4.5 | 1.13 × 10−10 |
| 841046 | AA486747 | Hs.8904 | Z391G | Ig superfamily protein | 4.4 | 2.03 × 10−9 |
| 502244 | AA133297 | — | — | ESTs | 3.8 | 2.30 × 10−6 |
| 470393 | AA031514 | Hs.2256 | MMP7 | Matrix metalloproteinase 7 | 3.2 | 1.28 × 10−6 |
| 292833 | N69227 | Hs.94953 | C1QG | Complement component 1 | 3.1 | 5.93 × 10−8 |
| 247587 | N58136 | — | — | ESTs | 2.4 | 1.65 × 10−6 |
| 73268 | T56021 | Hs.5057 | CPD | Carboxypeptidase D | 2.0 | 1.89 × 10−6 |
| 50182 | H17883 | — | — | ESTs | 1.8 | 9.72 × 10−7 |
| 813614 | AA447684 | Hs.1076 | SPRR1B | Small proline-rich protein 1B (cornifin) | 1.8 | 7.66 × 10−7 |
| 784360 | AA447196 | Hs.12451 | EML1 | Microtubule-associated protein | 1.7 | 1.13 × 10−6 |
| 324437 | W46900 | Hs.789 | CXCL1 | CXCL1 (GRO1 oncogene) | 1.6 | 7.09 × 10−7 |
| 739625 | AA479623 | Hs.227489 | SAST | Syntrophin-associated serine/threonine kinase | 1.6 | 5.21 × 10−7 |
| 382654 | AA069517 | — | — | ESTs | 1.6 | 3.22 × 10−6 |
| 786069 | AA448660 | Hs.49349 | BACE | Beta-site APP-cleaving enzyme | 0.7 | 2.53 × 10−6 |
| 130057 | R20779 | Hs.155223 | STC2 | Stanniocalcin 2 | 0.6 | 2.26 × 10−6 |
| 825785 | AA505117 | — | — | ESTs | 0.6 | 2.53 × 10−6 |
| 325641 | W51985 | Hs.251850 | PSG1 | Pregnancy-specific beta-1-glycoprotein | 0.5 | 7.40 × 10−7 |
| 811785 | AA463463 | — | — | ESTs | 0.5 | 7.94 × 10−7 |
| 199635 | R96522 | — | — | ESTs | 0.5 | 3.71 × 10−7 |
Clone ID . | Accession . | Cluster ID . | Name . | Annotation . | Ratio* . | P† . |
|---|---|---|---|---|---|---|
| 378461 | AA775616 | Hs.313 | SPP1 | Osteopontin | 15.4 | 1.63 × 10−6 |
| 418422 | W92812 | Hs.2164 | PPBP | CXCL7 | 13.7 | 2.69 × 10−9 |
| 840493 | AA485893 | Hs.78224 | RNASE1 | Ribonuclease, RNase A family | 7.6 | 4.36 × 10−8 |
| 773330 | AA425450 | — | — | ESTs | 4.5 | 1.13 × 10−10 |
| 841046 | AA486747 | Hs.8904 | Z391G | Ig superfamily protein | 4.4 | 2.03 × 10−9 |
| 502244 | AA133297 | — | — | ESTs | 3.8 | 2.30 × 10−6 |
| 470393 | AA031514 | Hs.2256 | MMP7 | Matrix metalloproteinase 7 | 3.2 | 1.28 × 10−6 |
| 292833 | N69227 | Hs.94953 | C1QG | Complement component 1 | 3.1 | 5.93 × 10−8 |
| 247587 | N58136 | — | — | ESTs | 2.4 | 1.65 × 10−6 |
| 73268 | T56021 | Hs.5057 | CPD | Carboxypeptidase D | 2.0 | 1.89 × 10−6 |
| 50182 | H17883 | — | — | ESTs | 1.8 | 9.72 × 10−7 |
| 813614 | AA447684 | Hs.1076 | SPRR1B | Small proline-rich protein 1B (cornifin) | 1.8 | 7.66 × 10−7 |
| 784360 | AA447196 | Hs.12451 | EML1 | Microtubule-associated protein | 1.7 | 1.13 × 10−6 |
| 324437 | W46900 | Hs.789 | CXCL1 | CXCL1 (GRO1 oncogene) | 1.6 | 7.09 × 10−7 |
| 739625 | AA479623 | Hs.227489 | SAST | Syntrophin-associated serine/threonine kinase | 1.6 | 5.21 × 10−7 |
| 382654 | AA069517 | — | — | ESTs | 1.6 | 3.22 × 10−6 |
| 786069 | AA448660 | Hs.49349 | BACE | Beta-site APP-cleaving enzyme | 0.7 | 2.53 × 10−6 |
| 130057 | R20779 | Hs.155223 | STC2 | Stanniocalcin 2 | 0.6 | 2.26 × 10−6 |
| 825785 | AA505117 | — | — | ESTs | 0.6 | 2.53 × 10−6 |
| 325641 | W51985 | Hs.251850 | PSG1 | Pregnancy-specific beta-1-glycoprotein | 0.5 | 7.40 × 10−7 |
| 811785 | AA463463 | — | — | ESTs | 0.5 | 7.94 × 10−7 |
| 199635 | R96522 | — | — | ESTs | 0.5 | 3.71 × 10−7 |
Identifiers and names are derived from information available in public databases, primarily the National Center for Biotechnology Information (NCBI) UniGene database and GenBank. Cultures were washed thoroughly to remove monocytes, and flow cytometry of the harvested cells showed 5% to 10% monocytes remaining in the cultures at 3 days.
— indicates that cluster ID or name is not available; ESTs, expressed sequence tags; and APP, amyloid precursor protein.
Linearized ratio of gene expression of cells in coculture to gene expression of HS-27a cells cultured alone. (Known monocyte-specific genes, such as HLA-DR, have been excluded.)
P value is based on a t test using point estimates by a Bayesian framework (CyberT) on 7 independent experiments.