Lymphoid tissue and cell subset analysis of nontransgenic and Bcl-2 transgenic adults
. | Nontransgenic . | Ln479 . | Nontransgenic . | Ln2 . |
|---|---|---|---|---|
| Body weight, g | 29.4 ± 5.7 | 29.4 ± 5.8 | 31.4 ± 4.0 | 32.0 ± 4.7 |
| Absolute cell numbers, × 107 | ||||
| Thymus | 7.7 ± 1.4 | 9.4 ± 1.1 | 10.8 ± 4.3 | 11.1 ± 3.6 |
| Spleen | 14.7 ± 1.6 | 31.9 ± 6.4 | 11.4 ± 1.1 | 37.3 ± 1.9 |
| Thymus cell subsets, % | ||||
| CD4-CD8- | 3.7 ± 1.0 | 4.6 ± 1.7 | 2.0, 3.8* | 5.1 ± 1.7 |
| CD4+CD8+ | 83.5 ± 3.6 | 75.6 ± 4.2 | 87.8, 83.3* | 70.9 ± 5.2 |
| CD4+CD8- | 9.9 ± 3.1 | 13.7 ± 2.0 | 11.9, 9.2* | 18.8 ± 2.4 |
| CD4-CD8+ | 3.0 ± 0.6 | 6.1 ± 0.7 | 1.0, 0.9* | 5.2 ± 1.4 |
| Spleen cell subsets, × 107 | ||||
| CD4+ | 3.1 ± 0.6 | 8.5 ± 1.1 | 2.8, 1.1* | 7.4 ± 1.9 |
| CD8+ | 1.6 ± 0.5 | 4.0 ± 0.3 | 1.0, 0.5* | 2.8 ± 1.6 |
| B220+ | 10.0 ± 0.7 | 21.4 ± 5.1 | 8.0, 2.9* | 18.6 ± 2.7 |
. | Nontransgenic . | Ln479 . | Nontransgenic . | Ln2 . |
|---|---|---|---|---|
| Body weight, g | 29.4 ± 5.7 | 29.4 ± 5.8 | 31.4 ± 4.0 | 32.0 ± 4.7 |
| Absolute cell numbers, × 107 | ||||
| Thymus | 7.7 ± 1.4 | 9.4 ± 1.1 | 10.8 ± 4.3 | 11.1 ± 3.6 |
| Spleen | 14.7 ± 1.6 | 31.9 ± 6.4 | 11.4 ± 1.1 | 37.3 ± 1.9 |
| Thymus cell subsets, % | ||||
| CD4-CD8- | 3.7 ± 1.0 | 4.6 ± 1.7 | 2.0, 3.8* | 5.1 ± 1.7 |
| CD4+CD8+ | 83.5 ± 3.6 | 75.6 ± 4.2 | 87.8, 83.3* | 70.9 ± 5.2 |
| CD4+CD8- | 9.9 ± 3.1 | 13.7 ± 2.0 | 11.9, 9.2* | 18.8 ± 2.4 |
| CD4-CD8+ | 3.0 ± 0.6 | 6.1 ± 0.7 | 1.0, 0.9* | 5.2 ± 1.4 |
| Spleen cell subsets, × 107 | ||||
| CD4+ | 3.1 ± 0.6 | 8.5 ± 1.1 | 2.8, 1.1* | 7.4 ± 1.9 |
| CD8+ | 1.6 ± 0.5 | 4.0 ± 0.3 | 1.0, 0.5* | 2.8 ± 1.6 |
| B220+ | 10.0 ± 0.7 | 21.4 ± 5.1 | 8.0, 2.9* | 18.6 ± 2.7 |
Tissues were isolated from mice ranging in age from 10 to 20 weeks. Both male and female mice are included in each of the groups. For the total thymus and spleen cell counts, sample numbers are as follows: Ln 479 mice, n = 6; their nontransgenic littermates, n = 6; Ln 2 mice, n = 11; their nontransgenic littermates, n = 7. For the spleen and thymus subset cell determinations, n = 3 except for nontransgenic Ln 2 control group. Values are given as mean ± SEM.
Data represent 2 values (n = 2)