Frequency analysis of HSCs in embryonic and adult hematopoietic tissues
Tissue transplanted . | Nontransgenic . | . | Bcl-2 trangenic . | . | ||
|---|---|---|---|---|---|---|
| . | HSC frequency . | HSC/ee . | HSC frequency . | HSC/ee . | ||
| E11 AGM | 1/2.7 × 105 | 0.37 | 1/0.6 × 105 | 1.70* | ||
| E12 AGM | 1/0.4 × 105 | 2.54 | 1/0.4 × 105 | 2.78 | ||
| E12 liver | 1/0.017 × 105 | 58.8 | 1/0.013 × 105 | 76.9 | ||
| Bone marrow | 1/6.28 × 104 | NA | 1/2.31 × 104 | NA | ||
Tissue transplanted . | Nontransgenic . | . | Bcl-2 trangenic . | . | ||
|---|---|---|---|---|---|---|
| . | HSC frequency . | HSC/ee . | HSC frequency . | HSC/ee . | ||
| E11 AGM | 1/2.7 × 105 | 0.37 | 1/0.6 × 105 | 1.70* | ||
| E12 AGM | 1/0.4 × 105 | 2.54 | 1/0.4 × 105 | 2.78 | ||
| E12 liver | 1/0.017 × 105 | 58.8 | 1/0.013 × 105 | 76.9 | ||
| Bone marrow | 1/6.28 × 104 | NA | 1/2.31 × 104 | NA | ||
HSC frequencies were determined from the primary transplantation data in Figure 6 and were calculated by means of L-Calc software (StemCell Technologies, Vancouver, BC, Canada). ee indicates embryo equivalent; and NA, not applicable.
Statistical significance; P < .02