Rescue of α-globin expression induced by truncated c-met mRNA injection by c-injection of full length of c-met mRNA
. | . | . | Decreased or absent . | . | |
|---|---|---|---|---|---|
| Dose full-length c-met* . | n . | Normal, % . | Anterior VBI, % . | Throughout VBI, % . | |
| 0 pg | 46 | 9 | 71 | 20 | |
| 2000 pg | 62 | 23 | 66 | 11 | |
| 4000 pg | 45 | 46 | 38 | 16 | |
. | . | . | Decreased or absent . | . | |
|---|---|---|---|---|---|
| Dose full-length c-met* . | n . | Normal, % . | Anterior VBI, % . | Throughout VBI, % . | |
| 0 pg | 46 | 9 | 71 | 20 | |
| 2000 pg | 62 | 23 | 66 | 11 | |
| 4000 pg | 45 | 46 | 38 | 16 | |
Frequency of globin expression pattern is given as a percentage. Embryos were scored for either the decrease of or absence in staining throughout the VBI, or the anterior portion of the VBI in embryos injected with mRNAs to dorsal-vegetable blastomeres.
The dose of truncated c-met used in these injections was 2000 pg.