Table 5. Selective HLA class I antigen... | American Society of Hematology

Table 5.

Selective HLA class I antigen loss identified by testing leukemic cells with HLA class I antigen-typing sera in CDC assay

Case no.	Leukemic subtype	Sample type	Normal cells							Tumor cells
			A	A	B	B	A	A	B	B
1	CLL	BL	10	31	38	40	NR^*	NR^*	38	40
2	AML	BL	24	31	60	H	24	31	60	H
3	CLL	BL	2	3	7	51	2	3	7	51
4	CLL	BL	1	2	5	8	1	2	5	8
5	CLL	BL	3	10	35	44	3	10	35	44
6	AML	BL	24	32	39	57	24	32	39	57
7	CLL	BL	2	10	8	14	2	10	NR^*	14
8	ALL	BL	2	3	60	H	2	3	60	H
9	CLL	BL	1	3	35	40	1	3	35	40
11	AML	BL	1	2	7	60	1	2	7	60
12	CLL	BL	3	23	7	44	3	23	7	44
13	CLL	BL	1	24	8	18	1	24	8	18
14	CLL	BL	24	32	35	51	24	32	35	51
15	AML	BL	ND	ND	ND	ND	3	11	35	38
16	CLL	BL	2	24	13	18	2	24	13	18
17	ALL	BL	1	H	5	7	1	H	5	7
18	ALL	BL	2	33	7	14	2	33	7	14
21	ALL	BL	ND	ND	ND	ND	10	28	52	H
23	AML	BL	2	32	8	17	2	32	8	17
26	CLL	BL	2	H	7	44	2	H	7	44
27	CLL	BL	2	H	8	44	2	H	8	44
28	CLL	BL	2	32	18	35	2	32	18	35
29	AML	BL	2	28	13	18	2	NR^*	13	18
30	AML	BL	2	H	17	42	2	H	17	42
31	CLL	BL	11	33	35	57	11	33	35	57
32	AML	BL	2	3	39	51	2	3	39	51
33	AML	BL	2	23	35	44	2	23	35	44
34	AML	BM	1	2	13	60	1	2	13	60
38	CLL	BL	1	2	8	12	NR^*	2	8	12
40	AML	BL	3	29	44	55	3	29	44	55
41	CLL	BL	3	29	51	60	3	29	51	60
42	CLL	BL	2	19	39	H	2	19	39	H
43	AML	BL	ND	ND	ND	ND	11	24	35	62
44	AML	BL	2	29	35	51	2	29	NR^*	51
46	AML	BL	ND	ND	ND	ND	3	25	22	35
47	CLL	BL	1	3	7	8	1	3	7	8
48	AML	BL	ND	ND	ND	ND	1	2	8	60

Case no.	Leukemic subtype	Sample type	Normal cells							Tumor cells
			A	A	B	B	A	A	B	B
1	CLL	BL	10	31	38	40	NR^*	NR^*	38	40
2	AML	BL	24	31	60	H	24	31	60	H
3	CLL	BL	2	3	7	51	2	3	7	51
4	CLL	BL	1	2	5	8	1	2	5	8
5	CLL	BL	3	10	35	44	3	10	35	44
6	AML	BL	24	32	39	57	24	32	39	57
7	CLL	BL	2	10	8	14	2	10	NR^*	14
8	ALL	BL	2	3	60	H	2	3	60	H
9	CLL	BL	1	3	35	40	1	3	35	40
11	AML	BL	1	2	7	60	1	2	7	60
12	CLL	BL	3	23	7	44	3	23	7	44
13	CLL	BL	1	24	8	18	1	24	8	18
14	CLL	BL	24	32	35	51	24	32	35	51
15	AML	BL	ND	ND	ND	ND	3	11	35	38
16	CLL	BL	2	24	13	18	2	24	13	18
17	ALL	BL	1	H	5	7	1	H	5	7
18	ALL	BL	2	33	7	14	2	33	7	14
21	ALL	BL	ND	ND	ND	ND	10	28	52	H
23	AML	BL	2	32	8	17	2	32	8	17
26	CLL	BL	2	H	7	44	2	H	7	44
27	CLL	BL	2	H	8	44	2	H	8	44
28	CLL	BL	2	32	18	35	2	32	18	35
29	AML	BL	2	28	13	18	2	NR^*	13	18
30	AML	BL	2	H	17	42	2	H	17	42
31	CLL	BL	11	33	35	57	11	33	35	57
32	AML	BL	2	3	39	51	2	3	39	51
33	AML	BL	2	23	35	44	2	23	35	44
34	AML	BM	1	2	13	60	1	2	13	60
38	CLL	BL	1	2	8	12	NR^*	2	8	12
40	AML	BL	3	29	44	55	3	29	44	55
41	CLL	BL	3	29	51	60	3	29	51	60
42	CLL	BL	2	19	39	H	2	19	39	H
43	AML	BL	ND	ND	ND	ND	11	24	35	62
44	AML	BL	2	29	35	51	2	29	NR^*	51
46	AML	BL	ND	ND	ND	ND	3	25	22	35
47	CLL	BL	1	3	7	8	1	3	7	8
48	AML	BL	ND	ND	ND	ND	1	2	8	60

Untouched leukemic cells and autologous lymphocytes were isolated using immunomagnetic procedure. HLA class I allospecificity expression was assessed by testing cells with HLA class I-typing sera in complement-dependent microcytotoxicity (CDC) assay.

H indicates a homozygous sample; ND, not done or not enough normal cells available; and NR, no reaction with the respective anti–HLA class I antisera.

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