Table 2.

Measurement of IVAPLA2 by titer on Western blots for the following preparations: U937 cells before and after affinity purification, red cells after affinity purification, enriched platelets, and enriched leukocytes

Cell preparation
Total cells used count, × 109/L
Total IVAPLA2 measured by titer, μg
Estimated IVAPLA2 cell concentration, fg/cell
U937 preaffinity preparation   1.22   356   290  
U937 postaffinity purification   NA   105*  NA  
RBC postaffinity purification   642   29.6   0.16† 
Platelets   18   50   2.8  
WBCs
 		 0.039
 		 < 0.62
 		 —‡
 
Cell preparation
Total cells used count, × 109/L
Total IVAPLA2 measured by titer, μg
Estimated IVAPLA2 cell concentration, fg/cell
U937 preaffinity preparation   1.22   356   290  
U937 postaffinity purification   NA   105*  NA  
RBC postaffinity purification   642   29.6   0.16† 
Platelets   18   50   2.8  
WBCs
 		 0.039
 		 < 0.62
 		 —‡
 

NA indicates not applicable.

*

This titer gives a recovery of 29% for the affinity process.

†

Assumes a 29% recovery of IVAPLA2 in the red cell affinity purification.

‡

This calculation is described in “Results and discussion.”

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