Phenotypic analysis of B cells infected with rF-TRICOM, activated with CD40L, or infected with rF-TRICOM and activated with CD40L; IFN-γ production by Flu-specific T cells using B cells as APCs
. | . | . | . | IFN-γ, pg/mL . | . | |
|---|---|---|---|---|---|---|
. | B7-1 . | ICAM-1 . | LFA-3 . | No peptide . | Flu peptide, 0.8 μg/mL . | |
| Uninfected | 6.9 (21.4) | 73.9 (22.0) | 79.6 (39.4) | < 15.6 | < 15.6 | |
| FP-WT | 8.4 (19.2) | 71.0 (32.3) | 82.7 (40.2) | < 15.6 | < 15.6 | |
| CD40L | 17.6 (29.8) | 86.6 (76.9) | 94.3 (86.8) | < 15.6 | 19.7 | |
| rF-TRICOM | 43.1 (51.5) | 82.4 (45.1) | 73.8 (47.2) | < 15.6 | 30.5 | |
| CD40L + rF-TRICOM | 46.7 (66.8) | 92.0 (90.2) | 96.0 (92.5) | < 15.6 | 59.7 | |
. | . | . | . | IFN-γ, pg/mL . | . | |
|---|---|---|---|---|---|---|
. | B7-1 . | ICAM-1 . | LFA-3 . | No peptide . | Flu peptide, 0.8 μg/mL . | |
| Uninfected | 6.9 (21.4) | 73.9 (22.0) | 79.6 (39.4) | < 15.6 | < 15.6 | |
| FP-WT | 8.4 (19.2) | 71.0 (32.3) | 82.7 (40.2) | < 15.6 | < 15.6 | |
| CD40L | 17.6 (29.8) | 86.6 (76.9) | 94.3 (86.8) | < 15.6 | 19.7 | |
| rF-TRICOM | 43.1 (51.5) | 82.4 (45.1) | 73.8 (47.2) | < 15.6 | 30.5 | |
| CD40L + rF-TRICOM | 46.7 (66.8) | 92.0 (90.2) | 96.0 (92.5) | < 15.6 | 59.7 | |
Numbers in the B7-1, ICAM-1, and LFA-3 columns indicate the percentage of positive cells for each surface marker, and the numbers in parentheses indicate mean fluorescence intensity (MFI). B cells from each treatment were used for stimulation of autologous CD8+ T cells (0.3 × 106/mL); supernatants were tested for IFN-γ production at 24 hours of stimulation. The APC/effector cell ratio used was 1:10.