IFN-γ production by PSA-specific T-cell lines stimulated with PSA(3A) peptide-pulsed autologous EBV-transformed B cells
. | IFN-γ production, pg/mL . | . | |
|---|---|---|---|
| T-cell line generation . | PSA(3A) . | No peptide . | |
| B cells infected with rF-TRICOM | 315.8 | < 15.6 | |
| B cells infected with rF-TRICOM + CD40L | 262.7 | < 15.6 | |
| B cells infected with rF-PSA(3A)/TRICOM | 1281.4 | < 15.6 | |
| Dendritic cells | 391.5 | 48.8 | |
. | IFN-γ production, pg/mL . | . | |
|---|---|---|---|
| T-cell line generation . | PSA(3A) . | No peptide . | |
| B cells infected with rF-TRICOM | 315.8 | < 15.6 | |
| B cells infected with rF-TRICOM + CD40L | 262.7 | < 15.6 | |
| B cells infected with rF-PSA(3A)/TRICOM | 1281.4 | < 15.6 | |
| Dendritic cells | 391.5 | 48.8 | |
Various PSA-specific T-cell lines were established using autologous B cells or DCs as APCs. Three IVS cycles were performed as described in “Materials and methods.” Results show IFN-γ production by the T cells (1 × 106/mL) at the fourth IVS, which was performed using autologous EBV-transformed B cells as APCs, pulsed with 25 μg/mL of PSA-3A peptide or without peptide. The APC/effector cell ratio was 2:1; supernatants were tested at 24 hours.