Expansion of bulk NK cells: donor HLA-C group 1 homozygous
Locus and concentration . | Expansion fold . | CD3-CD56+ or CD3-CD16+, % . | CD3-CD56+CD158b+, % . |
|---|---|---|---|
| HLA-Cw locus of feeder cells | |||
| Homozygous for C-G2 | (1.8 ± 1.6) × 104 | 79.5 ± 5.2 | 41 ± 9.1† |
| Homozygous for C-G1 (autologous) | (1.32 ± 0.87) × 104 | 72.5 ± 11.6 | 24 ± 8.8† |
| Heterozygous for C-G2 and C-G1 | (1.8 ± 0.45) × 104 | 78.6 ± 4.5 | 33 ± 9.5 |
| IL-2 concentration, IU/mL* | |||
| 100 | 2.6 × 104 | 76.6 | 35.5 |
| 50 | 2.5 × 104 | 75.7 | 27.2 |
| 5 | 6.8 × 103 | 68.2 | 21.8 |
Locus and concentration . | Expansion fold . | CD3-CD56+ or CD3-CD16+, % . | CD3-CD56+CD158b+, % . |
|---|---|---|---|
| HLA-Cw locus of feeder cells | |||
| Homozygous for C-G2 | (1.8 ± 1.6) × 104 | 79.5 ± 5.2 | 41 ± 9.1† |
| Homozygous for C-G1 (autologous) | (1.32 ± 0.87) × 104 | 72.5 ± 11.6 | 24 ± 8.8† |
| Heterozygous for C-G2 and C-G1 | (1.8 ± 0.45) × 104 | 78.6 ± 4.5 | 33 ± 9.5 |
| IL-2 concentration, IU/mL* | |||
| 100 | 2.6 × 104 | 76.6 | 35.5 |
| 50 | 2.5 × 104 | 75.7 | 27.2 |
| 5 | 6.8 × 103 | 68.2 | 21.8 |
NK cells were enriched by negative selection from PBMCs of a healthy donor (KR0350) homozygous for HLA-C group 1 (C-G1), then expanded in vitro using irradiated EBV-LCL feeders: (1) HLA-C group 2 (C-G2) EBV-LCLs; (2) autologous EBV-LCLs; (3) HLA-C group is heterozygous EBV-LCLs. Two weeks later, expanded NK cells were counted, and phenotype analyses were performed. The difference in CD3-CD56+CD158b+ population between homozygous for C-G2 feeder cells and autologous feeder cells was significant (P = .0085). Values represent averages ± SD from 3 independent experiments.
Values represent averages from 2 independent experiments.
P = .0085.