Effect of 1-nM Ang II intraperitoneal injection on P-selectin, E-selectin, VCAM-1, and ICAM-1 expression in the rat mesenteric arterioles and postcapillary venules
. | Arteriole, % . | . | Venule, % . | . | ||
|---|---|---|---|---|---|---|
| CAM . | Saline . | Ang II . | Saline . | Ang II . | ||
| P-selectin | 6.7 ± 4.2 | 66.7 ± 6.7* | 10.0 ± 4.5 | 70.0 ± 11.3* | ||
| E-selectin | 3.3 ± 3.3 | 23.3 ± 6.1† | 3.3 ± 3.3 | 36.7 ± 6.1* | ||
| VCAM-1 | 6.7 ± 4.2 | 56.7 ± 12.0* | 6.7 ± 4.2 | 63.3 ± 6.1* | ||
| ICAM-1 | 26.7 ± 4.2 | 50.0 ± 8.5† | 40.0 ± 7.3 | 63.3 ± 14.6† | ||
. | Arteriole, % . | . | Venule, % . | . | ||
|---|---|---|---|---|---|---|
| CAM . | Saline . | Ang II . | Saline . | Ang II . | ||
| P-selectin | 6.7 ± 4.2 | 66.7 ± 6.7* | 10.0 ± 4.5 | 70.0 ± 11.3* | ||
| E-selectin | 3.3 ± 3.3 | 23.3 ± 6.1† | 3.3 ± 3.3 | 36.7 ± 6.1* | ||
| VCAM-1 | 6.7 ± 4.2 | 56.7 ± 12.0* | 6.7 ± 4.2 | 63.3 ± 6.1* | ||
| ICAM-1 | 26.7 ± 4.2 | 50.0 ± 8.5† | 40.0 ± 7.3 | 63.3 ± 14.6† | ||
Rats were treated intraperitoneally with saline or 1 nM Ang II. Four hours later, the exposed mesentery was isolated and further fixed in paraformaldehyde. Immunohistochemical localization of P-selectin, E-selectin, ICAM-1, and VCAM-1 was accomplished using a modified avidin and biotin immunoperoxidase technique. Positive staining was defined as an arteriole or a venule displaying brown reaction product. Results are representative of 6 experiments for each treatment and are expressed as percentages of CAM expression (mean ± SEM).
P < .01.
P < .05 relative to the saline group.