Table 3. Discriminating polypeptide... | American Society of Hematology

Table 3.

Discriminating polypeptide patterns for GVHD and sepsis

Polypeptide identification							Frequency by group
No.	Mass, kDa	Time, min	Marker type	GVHD, %	No problems, %	Sepsis, %	GVHD	No problems	Sepsis
1	1.97	34.7	GVHD	82	18	92	801	89	253
2	1.75	36.0	GVHD	55	0	75	280	0	231
3	1.85	34.0	GVHD	64	9	33	314	28	73
4	1.07	37.8	GVHD	50	0	17	434	0	261
5	1.57	36.0	GVHD	59	9	83	246	25	650
6	1.87	46.3	GVHD	50	0	33	108	0	113
7	4.00	30.2	GVHD	50	0	8	842	0	103
8	1.83	33.2	GVHD	95	73	75	16 298	2047	5552
9	2.38	34.6	GVHD	18	73	17	53	301	89
10	3.44	44.1	GVHD	45	100	17	4581	12 182	169
11	1.35	48.1	GVHD	23	82	42	173	1572	76
12	3.21	33.6	GVHD	23	82	42	825	3605	1300
13	6.19	38.8	GVHD	23	82	8	275	2161	41
14	1.73	50.1	GVHD	32	91	25	344	799	138
15	3.09	45.9	GVHD	27	91	0	283	974	0
16	1.85	56.6	GVHD	9	82	25	5	650	19
17	1.54	50.7	Sepsis	18	9	83	33	7	267
18	2.17	42.1	Sepsis	18	9	83	114	97	604
19	1.24	32.4	Sepsis	23	27	92	141	227	549
20	2.05	46.2	Sepsis	18	18	83	38	46	169
21	2.15	41.6	Sepsis	36	36	100	877	290	1948
22	1.10	46.0	Sepsis	23	18	83	108	102	330
23	1.60	39.6	Sepsis	27	9	83	304	28	534
24	1.81	40.6	Sepsis	32	55	100	255	217	878
25	1.85	51.6	Sepsis	27	36	92	87	464	306
26	3.00	47.2	Sepsis	59	64	0	240	877	0
27	3.39	37.0	Sepsis	55	73	0	775	1348	0
28	3.84	25.8	Sepsis	59	73	0	3635	2219	0
29	4.04	29.2	Sepsis	59	73	0	1751	1405	0

Polypeptide identification							Frequency by group
No.	Mass, kDa	Time, min	Marker type	GVHD, %	No problems, %	Sepsis, %	GVHD	No problems	Sepsis
1	1.97	34.7	GVHD	82	18	92	801	89	253
2	1.75	36.0	GVHD	55	0	75	280	0	231
3	1.85	34.0	GVHD	64	9	33	314	28	73
4	1.07	37.8	GVHD	50	0	17	434	0	261
5	1.57	36.0	GVHD	59	9	83	246	25	650
6	1.87	46.3	GVHD	50	0	33	108	0	113
7	4.00	30.2	GVHD	50	0	8	842	0	103
8	1.83	33.2	GVHD	95	73	75	16 298	2047	5552
9	2.38	34.6	GVHD	18	73	17	53	301	89
10	3.44	44.1	GVHD	45	100	17	4581	12 182	169
11	1.35	48.1	GVHD	23	82	42	173	1572	76
12	3.21	33.6	GVHD	23	82	42	825	3605	1300
13	6.19	38.8	GVHD	23	82	8	275	2161	41
14	1.73	50.1	GVHD	32	91	25	344	799	138
15	3.09	45.9	GVHD	27	91	0	283	974	0
16	1.85	56.6	GVHD	9	82	25	5	650	19
17	1.54	50.7	Sepsis	18	9	83	33	7	267
18	2.17	42.1	Sepsis	18	9	83	114	97	604
19	1.24	32.4	Sepsis	23	27	92	141	227	549
20	2.05	46.2	Sepsis	18	18	83	38	46	169
21	2.15	41.6	Sepsis	36	36	100	877	290	1948
22	1.10	46.0	Sepsis	23	18	83	108	102	330
23	1.60	39.6	Sepsis	27	9	83	304	28	534
24	1.81	40.6	Sepsis	32	55	100	255	217	878
25	1.85	51.6	Sepsis	27	36	92	87	464	306
26	3.00	47.2	Sepsis	59	64	0	240	877	0
27	3.39	37.0	Sepsis	55	73	0	775	1348	0
28	3.84	25.8	Sepsis	59	73	0	3635	2219	0
29	4.04	29.2	Sepsis	59	73	0	1751	1405	0

Shown here are 16 polypeptides forming the diagnostic pattern (marker type: GVHD) with their frequency of appearance in groups of patients after HSCT (no “Other complications” as noted in Table 1), patients with GVHD (GVHD), and patients with sepsis. Thirteen additional polypeptides forming the “sepsis” pattern raise the discriminatory value of the GVHD panel, allowing differentiation of patients with sepsis from those with GVHD with 97% specificity and 100% sensitivity. The mean amplitude of signal intensity is shown for all groups.

View Large

This Feature Is Available To Subscribers Only