Nomenclature of parental and transduced Jurkat cell lines
Parental cells . | Introduced gene . | Nomenclature . | Mean CD3 expression ± standard deviation* . |
|---|---|---|---|
| P116 | — | ZAP-/Syk- | 440 ± 79 |
| P116 | ZAP | ZAPE/Syk- | 401 ± 84 |
| P116 | Syk | ZAP-/SykE | 392 ± 54 |
| E6.1 | Syk | ZAP+/SykE | 462 ± 91 |
| 77-6.8 | — | ZAP+/Syk+ | 234 ± 64 |
Parental cells . | Introduced gene . | Nomenclature . | Mean CD3 expression ± standard deviation* . |
|---|---|---|---|
| P116 | — | ZAP-/Syk- | 440 ± 79 |
| P116 | ZAP | ZAPE/Syk- | 401 ± 84 |
| P116 | Syk | ZAP-/SykE | 392 ± 54 |
| E6.1 | Syk | ZAP+/SykE | 462 ± 91 |
| 77-6.8 | — | ZAP+/Syk+ | 234 ± 64 |
+ indicates endogenous expression; E, ectopic expression; and —, no expression.
Mean fluorescence intensity of CD3 expression as monitored by flow cytometry using an αCD3 mAb, mean ± standard deviation in 5 independent experiments.