Expansion of CD94/NKG2A-expressing cells from paired PreG- and G-PBMC
. | Before, mean absolute no. cells ± × 106 . | . | After stimulation, mean absolute no. cells ± × 106 (mean fold expression) . | . | ||
|---|---|---|---|---|---|---|
| Surface marker . | PreG . | G-PBMC . | PreG . | G-PBMC . | ||
| CD94+/CD3+ | 0.048 ± 0.026 | 0.041 ± 0.012 | 2.87 ± 1.38 (59.8) | 5.51 ± 2.62* (134.4) | ||
| CD94+/CD8+ | 0.020 ± 0.007 | 0.010 ± 0.004 | 2.63 ± 1.25 (131.5) | 5.20 ± 2.36† (520.0) | ||
| NKG2A+/CD3+ | 0.021 ± 0.012 | 0.018 ± 0.010 | 2.09 ± 1.10 (99.5) | 4.20 ± 2.44* (233.3) | ||
| NKG2A+/CD8+ | 0.0052 ± 0.0036 | 0.0036 ± 0.0012 | 1.90 ± 1.02 (365.4) | 3.98 ± 2.21* (1105.6) | ||
. | Before, mean absolute no. cells ± × 106 . | . | After stimulation, mean absolute no. cells ± × 106 (mean fold expression) . | . | ||
|---|---|---|---|---|---|---|
| Surface marker . | PreG . | G-PBMC . | PreG . | G-PBMC . | ||
| CD94+/CD3+ | 0.048 ± 0.026 | 0.041 ± 0.012 | 2.87 ± 1.38 (59.8) | 5.51 ± 2.62* (134.4) | ||
| CD94+/CD8+ | 0.020 ± 0.007 | 0.010 ± 0.004 | 2.63 ± 1.25 (131.5) | 5.20 ± 2.36† (520.0) | ||
| NKG2A+/CD3+ | 0.021 ± 0.012 | 0.018 ± 0.010 | 2.09 ± 1.10 (99.5) | 4.20 ± 2.44* (233.3) | ||
| NKG2A+/CD8+ | 0.0052 ± 0.0036 | 0.0036 ± 0.0012 | 1.90 ± 1.02 (365.4) | 3.98 ± 2.21* (1105.6) | ||
Cultures were started from 2.5 × 106 mononuclear cells. Values indicate absolute number of cells before and after culture. Significant differences were noted when comparing the value of PreG and G-PBMC after stimulation (*P < .05, †P < .01).