Table 1. Maintenance of HCMV genomes... | American Society of Hematology

Table 1.

Maintenance of HCMV genomes during long-term culture

	No. HCMV genome equivalents*
Cell phenotype†	3 d after infection	10 d after infection	15 d after infection	20 d after infection	25 d after infection	33 d after infection
Experiment 1
Mock CD34⁺/CD38^-	0.001	0.003	0	0	0	0
Infected CD34⁺/CD38^-	0.974	62.119	6.976	0.449	0.042	0.005
Mock CD34⁺/c-kit⁺	0.005	0	0	0	0	0
Infected CD34⁺/c-kit⁺	0.716	1.741	0.222	0.002	0.001	0.001
Experiment 2
Mock CD34⁺/CD38^-	0.001	—	—	—	—	—
Infected CD34⁺/CD38^-	1.693	2.634	0.021	—	0.011	0.002
Infected CD34⁺/c-kit⁺	0.614	0.057	0.004	—	0.001	0

	No. HCMV genome equivalents*
Cell phenotype†	3 d after infection	10 d after infection	15 d after infection	20 d after infection	25 d after infection	33 d after infection
Experiment 1
Mock CD34⁺/CD38^-	0.001	0.003	0	0	0	0
Infected CD34⁺/CD38^-	0.974	62.119	6.976	0.449	0.042	0.005
Mock CD34⁺/c-kit⁺	0.005	0	0	0	0	0
Infected CD34⁺/c-kit⁺	0.716	1.741	0.222	0.002	0.001	0.001
Experiment 2
Mock CD34⁺/CD38^-	0.001	—	—	—	—	—
Infected CD34⁺/CD38^-	1.693	2.634	0.021	—	0.011	0.002
Infected CD34⁺/c-kit⁺	0.614	0.057	0.004	—	0.001	0

— indicates not done.

HCMV genome equivalents per cell were determined by generating standard curves using HCMV genomic DNA and cellular genomic DNA. Two independent experiments are shown. The standard curve for actin detected 10² to 10⁵ cell equivalents per reaction with correlation coefficients of 0.998 and 0.997, slopes of -3.45 and -3.44, and y intercept values of 36.69 and 36.76 for experiment 1 and 2, respectively. The standard curve for UL123 detected 10¹ to 10⁶ genome equivalents per reaction with correlation coefficients of 0.999 and 0.996, slopes of -3.39 and -3.45, and y-intercept values of 36.48 and 34.52 for experiment 1 and 2, respectively.

†

Each cell population was isolated by fluorescence-activated cell sorting with purities greater than 97%.

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