T2 Kd, Dd and Ld binding assay
. | Mean fluorescence intensity ratio* . | . | . | ||
|---|---|---|---|---|---|
| Peptide . | T2Kd . | T2Dd . | T2Ld . | ||
| DMSO | 1.0 | 1.0 | 1.0 | ||
| D9L | 1.9 | 1.0 | 1.0 | ||
| A9T | 1.2 | 1.0 | 1.0 | ||
| K9I | 1.0 | 1.4 | 1.1 | ||
| Y9Y | 1.2 | 1.2 | 1.0 | ||
| T9L | 1.0 | 1.3 | 1.0 | ||
| S9L | 1.0 | 1.3 | 1.0 | ||
| K9T | 1.2 | 1.1 | 1.0 | ||
| N9Y | 1.2 | 1.1 | 1.0 | ||
| P876 (β-gal peptide) | 1.0 | 1.9 | 2.9 | ||
. | Mean fluorescence intensity ratio* . | . | . | ||
|---|---|---|---|---|---|
| Peptide . | T2Kd . | T2Dd . | T2Ld . | ||
| DMSO | 1.0 | 1.0 | 1.0 | ||
| D9L | 1.9 | 1.0 | 1.0 | ||
| A9T | 1.2 | 1.0 | 1.0 | ||
| K9I | 1.0 | 1.4 | 1.1 | ||
| Y9Y | 1.2 | 1.2 | 1.0 | ||
| T9L | 1.0 | 1.3 | 1.0 | ||
| S9L | 1.0 | 1.3 | 1.0 | ||
| K9T | 1.2 | 1.1 | 1.0 | ||
| N9Y | 1.2 | 1.1 | 1.0 | ||
| P876 (β-gal peptide) | 1.0 | 1.9 | 2.9 | ||
Mean fluorescence intensity (MFI) ratio = (MFI peptide/MFI DMSO).