Table 1. Analysis of ALDH hi Lin − and... | American Society of Hematology

Table 1.

Analysis of ALDH^hiLin⁻ and ALDH^loLin⁻ cells for expression of primitive cell surface markers

	Frequency of expression, %
Cell type	Lin⁻	ALDH^loLin⁻	ALDH^hiLin⁻	P
CD34	59.5 ± 6.4	37.8 ± 7.2	91.0 ± 2.9	< .001
CD133	34.6 ± 5.5	11.5 ± 4.5	72.9 ± 4.2	< .001
CD31	86.4 ± 2.8	66.7 ± 6.4	97.9 ± 0.8	.001
CD117	61.2 ± 7.0	41.5 ± 7.1	83.1 ± 2.7	< .001
CD34⁺CD38⁻	13.8 ± 1.7	5.0 ± 1.3	25.5 ± 3.7	.002
CD34+CD133+	41.1 ± 6.0	12.0 ± 3.1	71.1 ± 4.6	< .001

	Frequency of expression, %
Cell type	Lin⁻	ALDH^loLin⁻	ALDH^hiLin⁻	P
CD34	59.5 ± 6.4	37.8 ± 7.2	91.0 ± 2.9	< .001
CD133	34.6 ± 5.5	11.5 ± 4.5	72.9 ± 4.2	< .001
CD31	86.4 ± 2.8	66.7 ± 6.4	97.9 ± 0.8	.001
CD117	61.2 ± 7.0	41.5 ± 7.1	83.1 ± 2.7	< .001
CD34⁺CD38⁻	13.8 ± 1.7	5.0 ± 1.3	25.5 ± 3.7	.002
CD34+CD133+	41.1 ± 6.0	12.0 ± 3.1	71.1 ± 4.6	< .001

Lineage-depleted human UCB MNCs were costained with Aldefluor and antibodies to various hematopoietic and primitive cell surface markers. The expression of each cell surface marker was enumerated and compared between the Lin⁻, ALDH^hiLin⁻, and ALDH^loLin⁻ populations as outlined in “Materials and methods.” The ALDH^hiLin⁻ cells demonstrated increased expression of CD45, CD34, CD133, CD31 (PECAM-1), and CD117 (c-kit) cell surface molecules in comparison to the ALDH^loLin⁻ cells. The ALDH^hiLin⁻ cells were also enriched for CD34⁺ CD38⁻ and CD34⁺CD133⁺ -expressing cells. Data represent mean ± SEM of 6 to 9 UCB samples, and P values were determined by Student t test comparing ALDH^loLin⁻ and ALDH^hiLin⁻ populations.

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