Human ALDHhiLin− cells are enriched for hematopoietic progenitors
. | No. of colonies per 103 cells . | . | . | . | Plating efficiency . | |||
|---|---|---|---|---|---|---|---|---|
| Cell population . | BFU-E . | CFU-MIX . | CFU-GM . | Total . | . | |||
| Lin− | 27.6 ± 5.4 | 2.4 ± 0.7 | 43.4 ± 5.3 | 73.4 ± 9.3 | 1 in 16.8 | |||
| ALDHloLin− | 0.2 ± 0.05 | 0.0 ± 0.0 | 0.3 ± 0.1 | 0.5 ± 0.1 | 1 in 3162 | |||
| ALDHhiLin− | 107.4 ± 10.1 | 10.7 ± 1.4 | 130.1 ± 14.8 | 248.3 ± 20.3 | 1 in 4.4 | |||
. | No. of colonies per 103 cells . | . | . | . | Plating efficiency . | |||
|---|---|---|---|---|---|---|---|---|
| Cell population . | BFU-E . | CFU-MIX . | CFU-GM . | Total . | . | |||
| Lin− | 27.6 ± 5.4 | 2.4 ± 0.7 | 43.4 ± 5.3 | 73.4 ± 9.3 | 1 in 16.8 | |||
| ALDHloLin− | 0.2 ± 0.05 | 0.0 ± 0.0 | 0.3 ± 0.1 | 0.5 ± 0.1 | 1 in 3162 | |||
| ALDHhiLin− | 107.4 ± 10.1 | 10.7 ± 1.4 | 130.1 ± 14.8 | 248.3 ± 20.3 | 1 in 4.4 | |||
Lineage depleted and purified ALDHhiLin− or ALDHloLin− cells were isolated and cultured in semisolid methylcellulose media (Methocult H4434) as described in “Materials and methods.” Human progenitor colonies of erythrocyte, granulocyte/macrophage and mixed lineages (BFU-E [erythroid burst-forming unit], CFU-GM [granulocyte-macrophage colony-forming unit], and CFU-MIX[mixed colony-forming unit]) were enumerated after 14 to 17 days of in vitro culture. Data represent the number of individual colonies produced per 1000 cells plated for doses ranging from 2 × 102 to 1 × 103 for ALDHhiLin− cells, 5 × 104 to 2 × 105 for ALDHloLin− cells, and 1 × 103 to 5 × 103 Lin− cells. Data are expressed as mean ± SEM for cells isolated from 4 to 5 fresh UCB samples (n = 4-5).