Table 1.

Survival as a result of EPO-tg

c-Kit
EPO-tg
No. mice
No. dead before day 20
No. surviving past day 20
W/W   −   4   4   0  
W/W   +   10   2   8  
W/+   −   14   0   14  
W/+
 		 +
 		 22
 		 0
 		 22
 
c-Kit
EPO-tg
No. mice
No. dead before day 20
No. surviving past day 20
W/W   −   4   4   0  
W/W   +   10   2   8  
W/+   −   14   0   14  
W/+
 		 +
 		 22
 		 0
 		 22
 

Fifty mice from mixed litters were monitored for survival beyond postnatal day 20 as a function of the presence of the EPO transgene. All mice were typed for c-Kit deficiency based on extreme pallor. The presence of the EPO transgene was determined using PCR analysis. Only c-KitW/W mice carrying the EPO-tg survived longer than 20 days. Also, in an independent experiment, 50 white (c-KitW/W) adult mice were tested for the presence of EPO-tg. Without exception, all 50 mice were positive for EPO-tg, indicating that survival strictly depends on its presence.

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