Number of mast cell colonies that developed after plating bone marrow, spleen, and peripheral blood mononuclear cells of WBB6F1-+/+ or WBB6F1-tg/tg mice in the presence of IL-3 alone or of both IL-3 and KitL
. | No. of colonies in each culture condition . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | IL-3* . | . | IL-3 + KitL† . | . | |||
| Cells . | WBB6F1-+/+ . | WBB6F1-tg/tg . | WBB6F1-+/+ . | WBB6F1-tg/tg . | |||
| Bone marrow | 2.8 ± 0.5 | 2.7 ± 0.5 | 8.0 ± 0.8 | 3.0 ± 0.5‡ | |||
| Spleen | 17.8 ± 5.4 | 11.5 ± 3.1 | 29.8 ± 6.1 | 10.0 ± 4.8‡ | |||
| Blood | 1.3 ± 0.5 | 0.8 ± 0.3 | 5.8 ± 1.4 | 1.0 ± 0.7‡ | |||
| BMMC | 4.3 ± 1.4 | 3.2 ± 0.9 | 16.2 ± 3.4 | 5.5 ± 0.8 | |||
. | No. of colonies in each culture condition . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | IL-3* . | . | IL-3 + KitL† . | . | |||
| Cells . | WBB6F1-+/+ . | WBB6F1-tg/tg . | WBB6F1-+/+ . | WBB6F1-tg/tg . | |||
| Bone marrow | 2.8 ± 0.5 | 2.7 ± 0.5 | 8.0 ± 0.8 | 3.0 ± 0.5‡ | |||
| Spleen | 17.8 ± 5.4 | 11.5 ± 3.1 | 29.8 ± 6.1 | 10.0 ± 4.8‡ | |||
| Blood | 1.3 ± 0.5 | 0.8 ± 0.3 | 5.8 ± 1.4 | 1.0 ± 0.7‡ | |||
| BMMC | 4.3 ± 1.4 | 3.2 ± 0.9 | 16.2 ± 3.4 | 5.5 ± 0.8 | |||
Values represent the mean ± SE of 6 mice (bone marrow, spleen, and blood) or of 3 cultures (BMMC) and are given as the number per 4 × 104 bone marrow cells, 2 × 105 spleen and peripheral blood mononuclear cells, and 5 × 102 CMCs.
Culture medium containing 10 ng/mL IL-3.
Culture medium containing 10 ng/mL IL-3 and 50 ng/mL KitL.
P < .01 by t test when compared with the value of WBB6F1-+/+ mice.